{"id":"5657d90c-08fe-5a1d-8d2f-bba0693d4149","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-isca-limited-turnover","predicate":"modestly_supports","statement":"ISCA1 and ISCA2 produced only limited enhancement of LIAS turnover in this donor comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dfd8f462-c61c-5187-9d3a-3845ef9ed0b3","mechanism_event_label":"Not every iron-sulfur carrier restores repeated activity equally well.","subject":{"id":"4717bf5e-52fa-50b8-a665-0a7239bbfe66","slug":"isca1","display_name":"Human ISCA1","entity_type_key":"protein"},"object":{"id":"be60e286-b472-584a-8f13-7050d0c3ad4f","slug":"lias-catalytic-turnover","display_name":"LIAS catalytic turnover","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dfd8f462-c61c-5187-9d3a-3845ef9ed0b3","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-isca-limited-turnover-event","event_type":"biochemical_relationship","label":"Not every iron-sulfur carrier restores repeated activity equally well.","description":"ISCA1 and ISCA2 produced only limited enhancement of LIAS turnover in this donor comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9db49c81-8504-51b8-95b8-8813f0d97497","slug":"isca2","display_name":"Human ISCA2","entity_type_key":"protein"},"role":"tested_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4717bf5e-52fa-50b8-a665-0a7239bbfe66","slug":"isca1","display_name":"Human ISCA1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"be60e286-b472-584a-8f13-7050d0c3ad4f","slug":"lias-catalytic-turnover","display_name":"LIAS catalytic turnover","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/36281303.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\", \"start_char\": 0, \"end_char\": 1732, \"text_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human LIAS turnover and cluster-transfer assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"LIAS with candidate iron-sulfur cluster donors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-free transfer distinguishes direct donor activity from upstream functions in intact cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human recombinant proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Not every iron-sulfur carrier restores repeated activity equally well.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p36281303] In Vitro Demonstration of Human Lipoyl Synthase Catalytic Activity in the Presence of NFU1. (2022). https://pubmed.ncbi.nlm.nih.gov/36281303/ DOI: 10.1021/acsbiomedchemau.2c00020","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial lipoyl synthesis machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bc3408dc-8c06-5a0a-95d0-5d2822a834a9","evidence_kind":"source_excerpt","locator":"Lines 351-362","start_line":351,"end_line":362,"excerpt":"### ala-isca-limited-turnover\nISCA1 and ISCA2 produced only limited enhancement of LIAS turnover in this donor comparison.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Not every iron-sulfur carrier restores repeated activity equally well.\norganism: Human recombinant proteins\ntissue_or_cell_type: Mitochondrial lipoyl synthesis machinery\nexperimental_model: Purified human LIAS turnover and cluster-transfer assays\nlimitations: Cell-free transfer distinguishes direct donor activity from upstream functions in intact cells.\nexposure: LIAS with candidate iron-sulfur cluster donors\nevidence_span: {\"source_cache\": \"artifacts/ala-research/36281303.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\", \"start_char\": 0, \"end_char\": 1732, \"text_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\"}\n[ala-p36281303] In Vitro Demonstration of Human Lipoyl Synthase Catalytic Activity in the Presence of NFU1. 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