{"id":"5623e692-0226-53dd-8932-4cbc6aa78264","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-aasdhppt-fasn","predicate":"donates_prosthetic_group_to","statement":"Human AASDHPPT transfers the 4′-phosphopantetheine moiety of CoA to a conserved serine in the human FASN acyl-carrier domain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8dc214d8-784d-549c-9119-31dff5a7cba5","mechanism_event_label":"CoA supplies the movable chemical arm used by the fatty-acid synthase carrier domain.","subject":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"object":{"id":"089297ff-6fdc-57e3-bad6-c59029957e64","slug":"fasn-holo-acp","display_name":"Human FASN phosphopantetheinylated ACP domain","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"8dc214d8-784d-549c-9119-31dff5a7cba5","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-aasdhppt-fasn-event","event_type":"biochemical_relationship","label":"CoA supplies the movable chemical arm used by the fatty-acid synthase carrier domain.","description":"Human AASDHPPT transfers the 4′-phosphopantetheine moiety of CoA to a conserved serine in the human FASN acyl-carrier domain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"df1ba694-d4ab-5a09-adac-272f9f9df60d","slug":"aasdhppt","display_name":"Human 4-phosphopantetheinyl transferase / AASDHPPT","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c56cf3ba-9e4a-5357-8645-969b8c4d6792","slug":"fasn","display_name":"Human fatty acid synthase / FASN","entity_type_key":"protein"},"role":"acceptor_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"transferred_moiety","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"089297ff-6fdc-57e3-bad6-c59029957e64","slug":"fasn-holo-acp","display_name":"Human FASN phosphopantetheinylated ACP domain","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract, substrate-specificity result","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human phosphopantetheinyl transferase and FASN ACP substrate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In-vitro enzymatic transfer from CoA; no dietary intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-limited extraction; catalytic activation is distinguished from measuring whole-cell fatty-acid flux. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CoA supplies the movable chemical arm used by the fatty-acid synthase carrier domain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. (2003). https://pubmed.ncbi.nlm.nih.gov/12815048/ DOI: 10.1074/jbc.m305459200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cytosolic FASN ACP domain","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e5eaa35-cd6e-5b7e-99ab-500737c2b25a","evidence_kind":"source_excerpt","locator":"Lines 795-807","start_line":795,"end_line":807,"excerpt":"### b5-met-aasdhppt-fasn\nHuman AASDHPPT transfers the 4′-phosphopantetheine moiety of CoA to a conserved serine in the human FASN acyl-carrier domain.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CoA supplies the movable chemical arm used by the fatty-acid synthase carrier domain.\norganism: Homo sapiens\ntissue_or_cell_type: Cytosolic FASN ACP domain\nexperimental_model: Recombinant human phosphopantetheinyl transferase and FASN ACP substrate\nlimitations: Abstract-limited extraction; catalytic activation is distinguished from measuring whole-cell fatty-acid flux. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: In-vitro enzymatic transfer from CoA; no dietary intervention.\ncross_nutrient: false\nevidence_location: Abstract, substrate-specificity result\n[b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. (2003). https://pubmed.ncbi.nlm.nih.gov/12815048/ DOI: 10.1074/jbc.m305459200","model_system":"Recombinant human phosphopantetheinyl transferase and FASN ACP substrate","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. (2003). https://pubmed.ncbi.nlm.nih.gov/12815048/ DOI: 10.1074/jbc.m305459200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}