{"id":"560165bd-6b0b-5b08-83f3-7e63db5ade91","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:plus-lariciresinol-permeabilises-bacterial-membranes","predicate":"reduces","statement":"(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c833efc1-1d0e-52a7-86b0-072479b4462e","mechanism_event_label":"(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.","subject":{"id":"09e8fc4d-e8f7-569b-a427-4c271f523a30","slug":"plus-lariciresinol","display_name":"(+)-Lariciresinol","entity_type_key":"small_molecule"},"object":{"id":"bc50ab1c-f5e9-565c-be45-6ca63d6d76b0","slug":"bacterial-membrane-integrity","display_name":"Bacterial membrane and cell wall integrity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c833efc1-1d0e-52a7-86b0-072479b4462e","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:plus-lariciresinol-permeabilises-bacterial-membranes-event","event_type":"observed_relationship","label":"(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.","description":"(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"09e8fc4d-e8f7-569b-a427-4c271f523a30","slug":"plus-lariciresinol","display_name":"(+)-Lariciresinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bc50ab1c-f5e9-565c-be45-6ca63d6d76b0","slug":"bacterial-membrane-integrity","display_name":"Bacterial membrane and cell wall integrity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Staphylococcus aureus KCTC1621 and Escherichia coli O157:H7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"(+)-Lariciresinol at minimum inhibitory concentration; MIC and minimum bactericidal concentration both 125 to 250 µg/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The active concentration is roughly 350 to 700 micromolar, two orders of magnitude above the concentrations at which the Nrf2 and enzyme mechanisms operate. Membrane permeabilisation at such concentrations is a low-selectivity mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Staphylococcus aureus KCTC1621 and Escherichia coli O157:H7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Efficacy of (+)-Lariciresinol to Control Bacterial Growth of Staphylococcus aureus and Escherichia coli O157:H7. (2017). https://pubmed.ncbi.nlm.nih.gov/28515721/ DOI: 10.3389/fmicb.2017.00804","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Bacterial membrane and cell wall integrity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7be3973c-929b-5be2-9023-72466fa50346","evidence_kind":"source_excerpt","locator":"Lines 231-240","start_line":231,"end_line":240,"excerpt":"## plus-lariciresinol-permeabilises-bacterial-membranes\n(+)-Lariciresinol at its minimum inhibitory concentration caused efflux of potassium ions and release of 260 nm absorbing material from Escherichia coli O157:H7 and Staphylococcus aureus, with deteriorated cell wall morphology on electron microscopy.\nModel/species: Staphylococcus aureus KCTC1621 and Escherichia coli O157:H7\nTissue/system: Bacterial membrane and cell wall integrity\nExposure: (+)-Lariciresinol at minimum inhibitory concentration; MIC and minimum bactericidal concentration both 125 to 250 µg/mL\nRoute: In vitro\nDuration: Not stated here\nLimits: The active concentration is roughly 350 to 700 micromolar, two orders of magnitude above the concentrations at which the Nrf2 and enzyme mechanisms operate. Membrane permeabilisation at such concentrations is a low-selectivity mechanism.\nPrimary reference: Efficacy of (+)-Lariciresinol to Control Bacterial Growth of Staphylococcus aureus and Escherichia coli O157:H7. (2017). https://pubmed.ncbi.nlm.nih.gov/28515721/ DOI: 10.3389/fmicb.2017.00804\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}