{"id":"55f63ed6-5400-50df-8504-b5cf5fd4f77b","stable_key":"195e8b84-ca5a-591a-a045-c55c53c8a01c:sodium-gut-loss","predicate":"loss_of_function_changes","statement":"Intestinal NHE3 deletion produced persistent watery, alkaline diarrhea.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6d9d8e80-183d-51ff-8fb3-1dd463873de6","mechanism_event_label":"A transport defect can create ongoing intestinal fluid losses.","subject":{"id":"c3e362a3-ead3-5b7b-9e6e-2a47fdb1cb72","slug":"mouse-slc9a3","display_name":"Mouse sodium/hydrogen exchanger NHE3 / Slc9a3","entity_type_key":"protein"},"object":{"id":"eb389c95-c416-51dd-81a6-39aa8c9fa6b9","slug":"intestinal-fluid-loss","display_name":"Intestinal fluid loss","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6d9d8e80-183d-51ff-8fb3-1dd463873de6","stable_key":"195e8b84-ca5a-591a-a045-c55c53c8a01c:sodium-gut-loss-event","event_type":"biochemical_relationship","label":"A transport defect can create ongoing intestinal fluid losses.","description":"Intestinal NHE3 deletion produced persistent watery, alkaline diarrhea.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c3e362a3-ead3-5b7b-9e6e-2a47fdb1cb72","slug":"mouse-slc9a3","display_name":"Mouse sodium/hydrogen exchanger NHE3 / Slc9a3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eb389c95-c416-51dd-81a6-39aa8c9fa6b9","slug":"intestinal-fluid-loss","display_name":"Intestinal fluid loss","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sodium-research/32227118.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0a9c8f006f6b0b8ff35bd7e67c529c9c72e0cf3d3fd29cd46ec8c23942129b39\", \"start_char\": 0, \"end_char\": 1643, \"text_sha256\": \"0a9c8f006f6b0b8ff35bd7e67c529c9c72e0cf3d3fd29cd46ec8c23942129b39\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Inducible intestinal epithelial Slc9a3 deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Tamoxifen-induced NHE3 loss","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic machinery failure, not isolated low sodium intake. Gut loss differs from proximal-tubule-specific deletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sodium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sodium","display_name":"Sodium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A transport defect can create ongoing intestinal fluid losses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sodium-p32227118] An inducible intestinal epithelial cell-specific NHE3 knockout mouse model mimicking congenital sodium diarrhea. (2020). https://pubmed.ncbi.nlm.nih.gov/32227118/ DOI: 10.1042/cs20200065","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestine with systemic electrolyte measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"31c072fe-ec7c-5463-a1c7-26b1157693b7","evidence_kind":"source_excerpt","locator":"Lines 824-835","start_line":824,"end_line":835,"excerpt":"### sodium-gut-loss\nIntestinal NHE3 deletion produced persistent watery, alkaline diarrhea.\nCondition category: machinery_impairment\nnutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A transport defect can create ongoing intestinal fluid losses.\norganism: Mouse\ntissue_or_cell_type: Intestine with systemic electrolyte measurements\nexperimental_model: Inducible intestinal epithelial Slc9a3 deletion\nlimitations: Genetic machinery failure, not isolated low sodium intake. Gut loss differs from proximal-tubule-specific deletion.\nexposure: Tamoxifen-induced NHE3 loss\nevidence_span: {\"source_cache\": \"artifacts/sodium-research/32227118.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0a9c8f006f6b0b8ff35bd7e67c529c9c72e0cf3d3fd29cd46ec8c23942129b39\", \"start_char\": 0, \"end_char\": 1643, \"text_sha256\": \"0a9c8f006f6b0b8ff35bd7e67c529c9c72e0cf3d3fd29cd46ec8c23942129b39\"}\n[sodium-p32227118] An inducible intestinal epithelial cell-specific NHE3 knockout mouse model mimicking congenital sodium diarrhea. (2020). https://pubmed.ncbi.nlm.nih.gov/32227118/ DOI: 10.1042/cs20200065","model_system":"Inducible intestinal epithelial Slc9a3 deletion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sodium-p32227118] An inducible intestinal epithelial cell-specific NHE3 knockout mouse model mimicking congenital sodium diarrhea. (2020). https://pubmed.ncbi.nlm.nih.gov/32227118/ DOI: 10.1042/cs20200065","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c260cd7b-beb0-5113-a198-69ec8fd4492c","stable_key":"import-195e8b84-ca5a-591a-a045-c55c53c8a01c","title":"Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"684f65cf0661c18395831ade4b02bb24d5e66c568f0d00a3471600eaf5d4d1c6","revision_id":"39866f04-3052-5f59-bd95-6806c5490875","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}