{"id":"558a5718-c5df-586c-9be0-ed2618b96c4d","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-nqo1-transcription","predicate":"increases_transcript_for","statement":"Boric acid increased NQO1 mRNA at measured time points within 1–4 hours in human DU-145 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4b718f3d-6c87-5358-a4a9-35bdf9a6709e","mechanism_event_label":"The cells increased instructions for NQO1; this does not measure the protein’s activity or a health benefit.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"01f73291-ab25-59e2-a5e9-71d15a6c2a0a","slug":"nqo1","display_name":"Human NAD(P)H quinone dehydrogenase 1 / NQO1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4b718f3d-6c87-5358-a4a9-35bdf9a6709e","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-nqo1-transcription-event","event_type":"biochemical_relationship","label":"The cells increased instructions for NQO1; this does not measure the protein’s activity or a health benefit.","description":"Boric acid increased NQO1 mRNA at measured time points within 1–4 hours in human DU-145 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"01f73291-ab25-59e2-a5e9-71d15a6c2a0a","slug":"nqo1","display_name":"Human NAD(P)H quinone dehydrogenase 1 / NQO1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/30196486.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e220df5fb0f9b8653035f7a144259ce66778467a7a72acbf7cd7d113f8b6030a\", \"start_char\": 0, \"end_char\": 2006, \"text_sha256\": \"e220df5fb0f9b8653035f7a144259ce66778467a7a72acbf7cd7d113f8b6030a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"PERK knockout comparison, immunofluorescence and quantitative PCR","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 µM boric acid; 1–6 hour comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Knockout dependence in mouse fibroblasts is separate from human tumor-cell transcription. Increased GCLC mRNA is not a measured increase in glutathione synthesis or clinical antioxidant benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cells increased instructions for NQO1; this does not measure the protein’s activity or a health benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p30196486] Boric Acid Activation of eIF2α and Nrf2 Is PERK Dependent: a Mechanism that Explains How Boron Prevents DNA Damage and Enhances Antioxidant Status. (2019). https://pubmed.ncbi.nlm.nih.gov/30196486/ DOI: 10.1007/s12011-018-1498-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"DU-145 prostate cancer cell culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b7566d11-7725-5b43-a53d-4ac27228df12","evidence_kind":"source_excerpt","locator":"Lines 547-558","start_line":547,"end_line":558,"excerpt":"### boron-nqo1-transcription\nBoric acid increased NQO1 mRNA at measured time points within 1–4 hours in human DU-145 cells.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The cells increased instructions for NQO1; this does not measure the protein’s activity or a health benefit.\norganism: Human\ntissue_or_cell_type: DU-145 prostate cancer cell culture\nexperimental_model: PERK knockout comparison, immunofluorescence and quantitative PCR\nlimitations: Knockout dependence in mouse fibroblasts is separate from human tumor-cell transcription. Increased GCLC mRNA is not a measured increase in glutathione synthesis or clinical antioxidant benefit.\nexposure: 10 µM boric acid; 1–6 hour comparisons\nevidence_span: {\"source_cache\": \"artifacts/boron-research/30196486.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e220df5fb0f9b8653035f7a144259ce66778467a7a72acbf7cd7d113f8b6030a\", \"start_char\": 0, \"end_char\": 2006, \"text_sha256\": \"e220df5fb0f9b8653035f7a144259ce66778467a7a72acbf7cd7d113f8b6030a\"}\n[boron-p30196486] Boric Acid Activation of eIF2α and Nrf2 Is PERK Dependent: a Mechanism that Explains How Boron Prevents DNA Damage and Enhances Antioxidant Status. (2019). https://pubmed.ncbi.nlm.nih.gov/30196486/ DOI: 10.1007/s12011-018-1498-4","model_system":"PERK knockout comparison, immunofluorescence and quantitative PCR","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p30196486] Boric Acid Activation of eIF2α and Nrf2 Is PERK Dependent: a Mechanism that Explains How Boron Prevents DNA Damage and Enhances Antioxidant Status. 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