{"id":"557208d9-dcd5-5448-9e2d-2aefb38933d7","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-bacterial-hydrolase-specificity","predicate":"does_not_transform","statement":"Recombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9b4c2163-24a5-5ce8-b88a-9ea0af563211","mechanism_event_label":"Recombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.","subject":{"id":"c2f09ddd-8c69-5754-87e2-904643f70bc9","slug":"eubacterium-phloretin-hydrolase","display_name":"Eubacterium ramulus phloretin hydrolase","entity_type_key":"protein"},"object":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9b4c2163-24a5-5ce8-b88a-9ea0af563211","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-bacterial-hydrolase-specificity-event","event_type":"observed_relationship","label":"Recombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.","description":"Recombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c2f09ddd-8c69-5754-87e2-904643f70bc9","slug":"eubacterium-phloretin-hydrolase","display_name":"Eubacterium ramulus phloretin hydrolase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"83949207-904f-53dc-bc9d-f7afaa546b34","slug":"phloretin","display_name":"Phloretin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"31594156-a5f2-5598-990b-ece19155ac6c","slug":"phloroglucinol","display_name":"Phloroglucinol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Phloretin or phlorizin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant bacterial enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The null for phlorizin separates glycoside hydrolysis from downstream aglycone cleavage; it does not exclude other microbial enzymes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phlorizin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Purified recombinant bacterial enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Recombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme. (2004). https://pubmed.ncbi.nlm.nih.gov/15466559/ DOI: 10.1128/AEM.70.10.6131-6137.2004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Substrate-specific carbon-carbon hydrolysis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8f9057f7-321f-59bb-befa-43679dd85ae5","evidence_kind":"source_excerpt","locator":"Lines 77-86","start_line":77,"end_line":86,"excerpt":"## phlorizin-bacterial-hydrolase-specificity\nRecombinant Eubacterium ramulus phloretin hydrolase cleaved phloretin but did not transform phlorizin.\nModel/species: Purified recombinant bacterial enzyme\nTissue/system: Substrate-specific carbon-carbon hydrolysis\nExposure: Phloretin or phlorizin\nRoute: In vitro\nDuration: Acute enzyme assay\nLimits: The null for phlorizin separates glycoside hydrolysis from downstream aglycone cleavage; it does not exclude other microbial enzymes.\nPrimary reference: Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme. (2004). https://pubmed.ncbi.nlm.nih.gov/15466559/ DOI: 10.1128/AEM.70.10.6131-6137.2004\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Purified recombinant bacterial enzyme","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7d4bfcb9-1665-5686-8b8a-1ad17ad6a4d2","stable_key":"import-bf161ddf-8b82-5d65-b86d-0b41245aac61","title":"Phlorizin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"13aa8f888801708147608784e74ad34d625b10fd76ea5afd542a3f9328bd40e2","revision_id":"6997ff77-8628-59b7-878c-95fed9d6a622","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}