{"id":"55581d47-3ef3-5bbd-99a7-bac9537a9f87","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-parent-efflux","predicate":"undergoes","statement":"Bidirectional permeability measurements supported active efflux of chlorogenic acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"74c74651-1a94-5706-b738-b7844bf8d42e","mechanism_event_label":"Movement back toward the gut side can limit passage across this cell model.","subject":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"object":{"id":"256d0f41-5e64-5a32-b7e9-b8f53c49e0ad","slug":"cga-caco2-efflux","display_name":"Caco-2 efflux of chlorogenic acid","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"74c74651-1a94-5706-b738-b7844bf8d42e","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-parent-efflux-event","event_type":"biochemical_relationship","label":"Movement back toward the gut side can limit passage across this cell model.","description":"Bidirectional permeability measurements supported active efflux of chlorogenic acid.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"256d0f41-5e64-5a32-b7e9-b8f53c49e0ad","slug":"cga-caco2-efflux","display_name":"Caco-2 efflux of chlorogenic acid","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/33336676.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3d5e4e2401998597110958f37fba3e510241ede894a037b6d0701be1689ea713\", \"start_char\": 0, \"end_char\": 1430, \"text_sha256\": \"3d5e4e2401998597110958f37fba3e510241ede894a037b6d0701be1689ea713\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bidirectional Caco-2 permeability assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chlorogenic acid and quinic acid; dosing details not provided in indexed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Active efflux is demonstrated at the assay level. A specific ABC transporter, gene knockout result or clinical drug interaction is not established by the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Movement back toward the gut side can limit passage across this cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chlorogenic_acid-p33336676] Demonstrating the involvement of an active efflux mechanism in the intestinal absorption of chlorogenic acid and quinic acid using a Caco-2 bidirectional permeability assay. (2021). https://pubmed.ncbi.nlm.nih.gov/33336676/ DOI: 10.1039/d0fo02629h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured intestinal epithelial barrier","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6fd4ad89-a816-5ceb-ab36-81230e55beca","evidence_kind":"source_excerpt","locator":"Lines 217-228","start_line":217,"end_line":228,"excerpt":"### chlorogenic_acid-parent-efflux\nBidirectional permeability measurements supported active efflux of chlorogenic acid.\nCondition category: normal\nnutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Movement back toward the gut side can limit passage across this cell model.\norganism: Human cell line\ntissue_or_cell_type: Cultured intestinal epithelial barrier\nexperimental_model: Bidirectional Caco-2 permeability assay\nlimitations: Active efflux is demonstrated at the assay level. A specific ABC transporter, gene knockout result or clinical drug interaction is not established by the abstract.\nexposure: Chlorogenic acid and quinic acid; dosing details not provided in indexed abstract\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/33336676.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3d5e4e2401998597110958f37fba3e510241ede894a037b6d0701be1689ea713\", \"start_char\": 0, \"end_char\": 1430, \"text_sha256\": \"3d5e4e2401998597110958f37fba3e510241ede894a037b6d0701be1689ea713\"}\n[chlorogenic_acid-p33336676] Demonstrating the involvement of an active efflux mechanism in the intestinal absorption of chlorogenic acid and quinic acid using a Caco-2 bidirectional permeability assay. 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