{"id":"55529174-4702-5bb5-863f-e78daddde408","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-cardiomyocyte-mitochondria","predicate":"reduces","statement":"Capsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cf0f3721-e673-572f-a313-3735afb3776d","mechanism_event_label":"Capsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.","subject":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"object":{"id":"fb63746e-aa83-5d67-9129-aacf04d3d78d","slug":"cardiomyocyte-mitochondrial-membrane-potential","display_name":"Cardiomyocyte mitochondrial membrane potential","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cf0f3721-e673-572f-a313-3735afb3776d","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-cardiomyocyte-mitochondria-event","event_type":"observed_relationship","label":"Capsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.","description":"Capsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fb63746e-aa83-5d67-9129-aacf04d3d78d","slug":"cardiomyocyte-mitochondrial-membrane-potential","display_name":"Cardiomyocyte mitochondrial membrane potential","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5e55433b-a2dc-5a10-a697-6824675fbc57","slug":"capsazepine","display_name":"Capsazepine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Capsaicin with capsazepine or cyclosporine controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary cardiomyocytes; rat reperfusion model for downstream peptide work","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The later infarct-size benefit was produced by the V1-cal peptide, not by capsaicin; capsaicin itself must not inherit that therapeutic result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Primary cardiomyocytes; rat reperfusion model for downstream peptide work","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Capsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transient Receptor Potential Vanilloid 1 Regulates Mitochondrial Membrane Potential and Myocardial Reperfusion Injury. (2016). https://pubmed.ncbi.nlm.nih.gov/27671317/ DOI: 10.1161/JAHA.116.003774","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Mitochondrial TRPV1 and membrane potential","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"952568b3-b8e0-5e20-b353-48e004ab0246","evidence_kind":"source_excerpt","locator":"Lines 121-130","start_line":121,"end_line":130,"excerpt":"## capsaicin-cardiomyocyte-mitochondria\nCapsaicin dose-dependently reduced mitochondrial membrane potential in primary cardiomyocytes, and capsazepine or cyclosporine blocked the effect.\nModel/species: Primary cardiomyocytes; rat reperfusion model for downstream peptide work\nTissue/system: Mitochondrial TRPV1 and membrane potential\nExposure: Capsaicin with capsazepine or cyclosporine controls\nRoute: In vitro\nDuration: Acute\nLimits: The later infarct-size benefit was produced by the V1-cal peptide, not by capsaicin; capsaicin itself must not inherit that therapeutic result.\nPrimary reference: Transient Receptor Potential Vanilloid 1 Regulates Mitochondrial Membrane Potential and Myocardial Reperfusion Injury. (2016). https://pubmed.ncbi.nlm.nih.gov/27671317/ DOI: 10.1161/JAHA.116.003774\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Primary cardiomyocytes; rat reperfusion model for downstream peptide work","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"883c8afb-af48-5561-ae3b-00d4f3c26752","stable_key":"import-49fecf36-4958-5f15-8c78-9a68cba50763","title":"Capsaicin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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