{"id":"55093b8f-f1ea-5003-adf4-81b6c2bad12b","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-gut-micronucleus-null","predicate":"no_detected_increase","statement":"Tartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"3abef0b9-9119-5b10-9a0e-7f87cbd12432","mechanism_event_label":"Tartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"ea63a8e7-f09e-58a8-b854-0d6e10bcbdaf","slug":"mouse-gut-micronucleus-frequency","display_name":"Mouse gut micronucleus frequency","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3abef0b9-9119-5b10-9a0e-7f87cbd12432","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-gut-micronucleus-null-event","event_type":"observed_relationship","label":"Tartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.","description":"Tartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ea63a8e7-f09e-58a8-b854-0d6e10bcbdaf","slug":"mouse-gut-micronucleus-frequency","display_name":"Mouse gut micronucleus frequency","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine doses up to 2000 mg/kg body weight","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Doses 24 h apart","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mice in the in vivo gut micronucleus assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No micronucleus increase under this protocol does not exclude transient DNA damage, other endpoints, or chronic effects. Co-studied dyes and their metabolites are not pooled as tartrazine-specific results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mice in the in vivo gut micronucleus assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lack of genotoxic effect of food dyes amaranth, sunset yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. (2009). https://pubmed.ncbi.nlm.nih.gov/19095036/ DOI: 10.1016/j.fct.2008.11.034","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral gavage twice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Intestinal genotoxicity endpoint","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3763a61e-c377-574d-be89-c2ae8f1e3419","evidence_kind":"source_excerpt","locator":"Lines 347-356","start_line":347,"end_line":356,"excerpt":"## tartrazine-gut-micronucleus-null\nTartrazine did not increase gut micronucleus frequency under the tested acute mouse protocol.\nModel/species: Mice in the in vivo gut micronucleus assay\nTissue: Intestinal genotoxicity endpoint\nExposure: Tartrazine doses up to 2000 mg/kg body weight\nRoute: Oral gavage twice\nDuration: Doses 24 h apart\nLimits: No micronucleus increase under this protocol does not exclude transient DNA damage, other endpoints, or chronic effects. Co-studied dyes and their metabolites are not pooled as tartrazine-specific results.\nPrimary reference: Lack of genotoxic effect of food dyes amaranth, sunset yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. (2009). https://pubmed.ncbi.nlm.nih.gov/19095036/ DOI: 10.1016/j.fct.2008.11.034\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Mice in the in vivo gut micronucleus assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}