{"id":"54eec44e-3ac3-5441-bfcc-7c12da2e2179","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:mouse-k-restriction-trabecular-bone","predicate":"restriction-changes","statement":"Prolonged low-potassium feeding reduced trabecular bone mineral density in the male-mouse study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"57894d81-5807-597c-992e-e1932daa5f0e","mechanism_event_label":"The mineral imbalance extended to a measured skeletal endpoint.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"006aa08b-b2cc-5aab-886d-04fdfe9f2947","slug":"trabecular-bone-mineral-density","display_name":"Trabecular bone mineral density","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"57894d81-5807-597c-992e-e1932daa5f0e","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:mouse-k-restriction-trabecular-bone-event","event_type":"biochemical_relationship","label":"The mineral imbalance extended to a measured skeletal endpoint.","description":"Prolonged low-potassium feeding reduced trabecular bone mineral density in the male-mouse study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"006aa08b-b2cc-5aab-886d-04fdfe9f2947","slug":"trabecular-bone-mineral-density","display_name":"Trabecular bone mineral density","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"affected-mineral","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e162b0ab-1449-5471-b3c3-4638a0fa3a96","slug":"pth","display_name":"Parathyroid hormone / PTH","entity_type_key":"protein"},"role":"measured-or-associated-hormone","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Dietary potassium restriction altered renal calcium balance, circulating PTH and trabecular mineral density; each endpoint is recorded independently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"Prolonged low-potassium feeding reduced trabecular bone mineral density in the male-mouse study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Male mice; two-week potassium-free discovery study and four/eight-week low-potassium study; urine/plasma minerals, renal proteomics, skeletal imaging, and constitutively active SPAK model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male mice; two-week potassium-free discovery study and four/eight-week low-potassium study; urine/plasma minerals, renal proteomics, skeletal imaging, and constitutively active SPAK model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Male mouse model; co-occurring renal transporter and endocrine changes do not prove a complete causal chain or a human fracture effect. NCC-specific mediation is incompletely isolated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mineral imbalance extended to a measured skeletal endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[murali-2026-calcium] Dietary potassium restriction causes hypercalciuria, hypocalcemia, and bone loss in male mice (2026). https://insight.jci.org/articles/view/196339 DOI: 10.1172/jci.insight.196339","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"kidney, blood and trabecular skeleton","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b9ff4128-6c46-5180-9b85-3754ea5c2154","evidence_kind":"source_excerpt","locator":"Lines 1330-1342","start_line":1330,"end_line":1342,"excerpt":"### mouse-k-restriction-trabecular-bone\nProlonged low-potassium feeding reduced trabecular bone mineral density in the male-mouse study.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The mineral imbalance extended to a measured skeletal endpoint.\norganism: Mus musculus\ntissue_or_cell_type: kidney, blood and trabecular skeleton\nexperimental_model: Male mice; two-week potassium-free discovery study and four/eight-week low-potassium study; urine/plasma minerals, renal proteomics, skeletal imaging, and constitutively active SPAK model.\nlimitations: Male mouse model; co-occurring renal transporter and endocrine changes do not prove a complete causal chain or a human fracture effect. NCC-specific mediation is incompletely isolated.\ncross_nutrient: Dietary potassium restriction altered renal calcium balance, circulating PTH and trabecular mineral density; each endpoint is recorded independently.\nexperimental-exposure: Male mice; two-week potassium-free discovery study and four/eight-week low-potassium study; urine/plasma minerals, renal proteomics, skeletal imaging, and constitutively active SPAK model.\nendpoint: Prolonged low-potassium feeding reduced trabecular bone mineral density in the male-mouse study.\n[murali-2026-calcium] Dietary potassium restriction causes hypercalciuria, hypocalcemia, and bone loss in male mice (2026). https://insight.jci.org/articles/view/196339 DOI: 10.1172/jci.insight.196339","model_system":"Male mice; two-week potassium-free discovery study and four/eight-week low-potassium study; urine/plasma minerals, renal proteomics, skeletal imaging, and constitutively active SPAK model.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [murali-2026-calcium] Dietary potassium restriction causes hypercalciuria, hypocalcemia, and bone loss in male mice (2026). https://insight.jci.org/articles/view/196339 DOI: 10.1172/jci.insight.196339","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}