{"id":"54e6b83f-87bc-5fce-8418-0eda68fbc1fa","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-liver-mito-transport","predicate":"drives","statement":"Agmatine entered rat liver mitochondrial matrix through membrane-potential-dependent transport; putrescine, arginine, ornithine and lysine did not inhibit uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"20e42dc4-f4c9-5197-abd4-720f283285bc","mechanism_event_label":"Mitochondrial entry has different requirements from plasma-membrane entry.","subject":{"id":"af606e41-0e0a-5abe-ad9d-7ab6ebce300d","slug":"rat-liver-mitochondrial-membrane-potential","display_name":"Rat liver mitochondrial membrane potential","entity_type_key":"cellular_process"},"object":{"id":"6b94bbcc-1a1b-5073-a1de-17fca5ec2d0f","slug":"rat-liver-mitochondrial-agmatine-uptake","display_name":"Rat liver mitochondrial agmatine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"20e42dc4-f4c9-5197-abd4-720f283285bc","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-liver-mito-transport-event","event_type":"observed_relationship","label":"Mitochondrial entry has different requirements from plasma-membrane entry.","description":"Agmatine entered rat liver mitochondrial matrix through membrane-potential-dependent transport; putrescine, arginine, ornithine and lysine did not inhibit uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"af606e41-0e0a-5abe-ad9d-7ab6ebce300d","slug":"rat-liver-mitochondrial-membrane-potential","display_name":"Rat liver mitochondrial membrane potential","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b94bbcc-1a1b-5073-a1de-17fca5ec2d0f","slug":"rat-liver-mitochondrial-agmatine-uptake","display_name":"Rat liver mitochondrial agmatine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat liver mitochondria; force-flux and inhibitor analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The proposed transporter was not genetically identified; no single universal agmatine carrier is asserted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Mitochondrial entry has different requirements from plasma-membrane entry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine is transported into liver mitochondria by a specific electrophoretic mechanism. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16509824/ · DOI 10.1042/BJ20060003","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b333c02b-247c-5a55-b973-79abc74f3d4a","evidence_kind":"source_excerpt","locator":"Lines 372-378","start_line":372,"end_line":378,"excerpt":"## agmatine-sulfate-liver-mito-transport\nMitochondrial entry has different requirements from plasma-membrane entry.\nAgmatine entered rat liver mitochondrial matrix through membrane-potential-dependent transport; putrescine, arginine, ornithine and lysine did not inhibit uptake.\nModel: Isolated rat liver mitochondria; force-flux and inhibitor analysis.\nLimitations: The proposed transporter was not genetically identified; no single universal agmatine carrier is asserted.\nEvidence access: Primary abstract\nAgmatine is transported into liver mitochondria by a specific electrophoretic mechanism. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16509824/ · DOI 10.1042/BJ20060003","model_system":"Isolated rat liver mitochondria; force-flux and inhibitor analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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