{"id":"54d5691e-ef60-5f20-a204-abc3fcef9c21","stable_key":"research:plp-supports-scly-catalysis","predicate":"supports","statement":"SCLY is a pyridoxal-phosphate-dependent enzyme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"ff219b1a-21ff-56ec-b6f9-c5358596579c","mechanism_event_label":"SCLY uses a vitamin B6-derived cofactor.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ff219b1a-21ff-56ec-b6f9-c5358596579c","stable_key":"research:plp-supports-scly-catalysis","event_type":"cofactor-dependence","label":"SCLY uses a vitamin B6-derived cofactor.","description":"SCLY is a pyridoxal-phosphate-dependent enzyme.","status":"active","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"liver-derived enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that extra vitamin B6 improves selenium recycling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d3aaa9b6-9d52-5188-b559-3ad8316556ec","evidence_kind":"curated_literature_summary","locator":"lines 522-532","start_line":522,"end_line":532,"excerpt":"## plp-supports-scly-catalysis\n\nSCLY uses a vitamin B6-derived cofactor.\n\nSCLY is a pyridoxal-phosphate-dependent enzyme.\n\nOrganism: mouse\nCell type: liver-derived enzyme\nExperimental model: Enzyme characterization\nLimitations: Not evidence that extra vitamin B6 improves selenium recycling.\nPrimary reference: [cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis](https://pubmed.ncbi.nlm.nih.gov/10692412/)","model_system":"Enzyme characterization","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"vitamin-cofactors","plain_language":"SCLY uses a vitamin B6-derived cofactor.","evidence_scope":"direct_experimental","papers":[{"key":"mihara-2000-scly","title":"cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis","url":"https://pubmed.ncbi.nlm.nih.gov/10692412/","doi":"10.1074/jbc.275.9.6195","year":2000,"model":"Purified mouse liver enzyme and recombinant characterization","summary":"Characterizes PLP-dependent selenocysteine decomposition by mammalian SCLY."}]}}