{"id":"54be81ec-2255-59f9-b8b9-335029cf565f","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:atic-aicar-formylation","predicate":"formylates","statement":"Purified human ATIC uses 10-formyl-THF for its AICAR formyltransferase reaction, yielding FAICAR.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cc81e137-e57c-5e38-9098-2f919db9144f","mechanism_event_label":"A second folate donation helps finish the purine ring.","subject":{"id":"9dec8ed2-6916-5967-8a8c-243dd6d4d5f1","slug":"atic","display_name":"Human bifunctional ATIC","entity_type_key":"protein"},"object":{"id":"4af06f63-38d1-5e5a-be55-69db44f251f7","slug":"aicar-ribotide","display_name":"5-Aminoimidazole-4-carboxamide ribonucleotide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cc81e137-e57c-5e38-9098-2f919db9144f","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:atic-aicar-formylation-event","event_type":"biochemical_relationship","label":"A second folate donation helps finish the purine ring.","description":"Purified human ATIC uses 10-formyl-THF for its AICAR formyltransferase reaction, yielding FAICAR.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"ec73b364-a2ea-5c4a-bcea-a54aeebf57d1","slug":"10-formyltetrahydrofolate","display_name":"10-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"formyl donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"469c873f-d969-5bda-afab-a2129a0d32f4","slug":"faicar","display_name":"5-Formamidoimidazole-4-carboxamide ribonucleotide","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9dec8ed2-6916-5967-8a8c-243dd6d4d5f1","slug":"atic","display_name":"Human bifunctional ATIC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4af06f63-38d1-5e5a-be55-69db44f251f7","slug":"aicar-ribotide","display_name":"5-Aminoimidazole-4-carboxamide ribonucleotide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reported folate kinetics used a 6R/6S mixture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second folate donation helps finish the purine ring.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0379aafa-cb79-50ee-a991-19e8bc69c611","evidence_kind":"source_excerpt","locator":"Lines 1010-1020","start_line":1010,"end_line":1020,"excerpt":"### atic-aicar-formylation\nPurified human ATIC uses 10-formyl-THF for its AICAR formyltransferase reaction, yielding FAICAR.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second folate donation helps finish the purine ring.\norganism: Homo sapiens\ntissue_or_cell_type: Cell-free\nexperimental_model: Recombinant enzyme kinetics\nlimitations: Reported folate kinetics used a 6R/6S mixture.\nexposure: Assay conditions described in the linked primary study.\n[rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","model_system":"Recombinant enzyme kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}