{"id":"54b7dee9-4b5e-561b-bc7f-f3f99fc8d082","stable_key":"cea4b417-bb71-5d5d-8fe1-4e175aca750c:graviola-complex1-atp","predicate":"inhibits_and_depletes","statement":"Annonacin inhibited mitochondrial complex-I-linked energy production, producing a concentration-dependent ATP fall, tau redistribution and death in cultured rat striatal neurons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4aff98e9-46b9-5832-99f0-b766a40fde11","mechanism_event_label":"Annonacin inhibited mitochondrial complex-I-linked energy production, producing a concentration-dependent ATP fall, tau redistribution and death in cultured rat striatal neurons.","subject":{"id":"46b657b6-1692-59cb-bccd-e94db35a2734","slug":"annonacin","display_name":"Annonacin","entity_type_key":"small_molecule"},"object":{"id":"7f16a467-c881-589c-bce1-ace07b163418","slug":"rat-neuronal-mitochondrial-complex1-atp","display_name":"Mitochondrial complex-I function and ATP in rat striatal neurons","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4aff98e9-46b9-5832-99f0-b766a40fde11","stable_key":"cea4b417-bb71-5d5d-8fe1-4e175aca750c:graviola-complex1-atp-event","event_type":"observed_relationship","label":"Annonacin inhibited mitochondrial complex-I-linked energy production, producing a concentration-dependent ATP fall, tau redistribution and death in cultured rat striatal neurons.","description":"Annonacin inhibited mitochondrial complex-I-linked energy production, producing a concentration-dependent ATP fall, tau redistribution and death in cultured rat striatal neurons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"46b657b6-1692-59cb-bccd-e94db35a2734","slug":"annonacin","display_name":"Annonacin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f16a467-c881-589c-bce1-ace07b163418","slug":"rat-neuronal-mitochondrial-complex1-atp","display_name":"Mitochondrial complex-I function and ATP in rat striatal neurons","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"dfb1483b-598f-5c9c-bfec-cc90ce2718e8","slug":"tau-protein","display_name":"Tau protein / MAPT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Annonacin concentration series","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"48 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2007). https://pubmed.ncbi.nlm.nih.gov/17634376/ DOI: 10.1523/JNEUROSCI.1644-07.2007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"ATP, mitochondrial transport, tau localization and cell death","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"08a15f97-fca8-5e4c-a78d-9f8a4dc0e927","evidence_kind":"source_excerpt","locator":"Lines 44-53","start_line":44,"end_line":53,"excerpt":"## graviola-complex1-atp\nAnnonacin inhibited mitochondrial complex-I-linked energy production, producing a concentration-dependent ATP fall, tau redistribution and death in cultured rat striatal neurons.\nModel/species: Primary rat striatal neurons\nTissue/system: ATP, mitochondrial transport, tau localization and cell death\nExposure: Annonacin concentration series\nRoute: In vitro\nDuration: 48 hours\nLimits: Purified-cell exposure establishes a hazard mechanism, not the dose delivered to human neurons by a particular graviola food.\nPrimary reference: Annonacin, a natural mitochondrial complex I inhibitor, causes tau pathology in cultured neurons. (2007). https://pubmed.ncbi.nlm.nih.gov/17634376/ DOI: 10.1523/JNEUROSCI.1644-07.2007\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Primary rat striatal neurons","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dde73deb-4328-5cc7-aa41-04b7d845f4fa","stable_key":"import-cea4b417-bb71-5d5d-8fe1-4e175aca750c","title":"Graviola (Annona muricata): mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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