{"id":"54965da0-e73e-5266-8874-6c25a158f820","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-alp-reaction","predicate":"hydrolyzes","statement":"Recombinant rat-brain agmatinase-like protein catalyzed agmatine hydrolysis; its central 210-residue construct retained activity with reduced kcat.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"321702b9-3c17-5494-8601-c8b352600e90","mechanism_event_label":"A distinct protein preparation can perform the reaction missing from purified human AGMAT.","subject":{"id":"a44bcaf9-a7c7-5d42-a850-6eb7b4fae03c","slug":"rat-agmatinase-like-protein","display_name":"Rat brain agmatinase-like protein / ALP experimental preparation","entity_type_key":"protein"},"object":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"321702b9-3c17-5494-8601-c8b352600e90","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-alp-reaction-event","event_type":"observed_relationship","label":"A distinct protein preparation can perform the reaction missing from purified human AGMAT.","description":"Recombinant rat-brain agmatinase-like protein catalyzed agmatine hydrolysis; its central 210-residue construct retained activity with reduced kcat.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a44bcaf9-a7c7-5d42-a850-6eb7b4fae03c","slug":"rat-agmatinase-like-protein","display_name":"Rat brain agmatinase-like protein / ALP experimental preparation","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"37cf0152-db25-5773-a726-d0c0facb2e24","slug":"urea","display_name":"Urea","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat ALP recombinant constructs and enzyme kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ALP is not interchangeable with the AGMAT gene product; no human oral exposure outcome is shown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A distinct protein preparation can perform the reaction missing from purified human AGMAT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Insights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32531922/ · DOI 10.3390/ijms21114132","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3d084b07-a1a3-5a38-bb1d-e7d6a472c390","evidence_kind":"source_excerpt","locator":"Lines 148-154","start_line":148,"end_line":154,"excerpt":"## agmatine-sulfate-rat-alp-reaction\nA distinct protein preparation can perform the reaction missing from purified human AGMAT.\nRecombinant rat-brain agmatinase-like protein catalyzed agmatine hydrolysis; its central 210-residue construct retained activity with reduced kcat.\nModel: Rat ALP recombinant constructs and enzyme kinetics.\nLimitations: ALP is not interchangeable with the AGMAT gene product; no human oral exposure outcome is shown.\nEvidence access: Primary abstract\nInsights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32531922/ · DOI 10.3390/ijms21114132","model_system":"Rat ALP recombinant constructs and enzyme kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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