{"id":"548d6af0-194b-5cb1-bc8c-b11ee8d54c9e","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-mixture-quenching","predicate":"quenches","statement":"The equal-ratio meso-zeaxanthin, zeaxanthin and lutein mixture quenched more singlet oxygen than each individual pigment at the same total concentration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2d98de70-03a6-504d-9fb2-2603668a358a","mechanism_event_label":"The three pigments worked better together in this solution experiment.","subject":{"id":"9bd3f7b4-0e76-5586-9eef-16e0b30865be","slug":"macular-carotenoid-111-mixture","display_name":"Meso-zeaxanthin, zeaxanthin and lutein 1:1:1 assay mixture","entity_type_key":"chemical_species"},"object":{"id":"ac4a9e3e-1454-5802-82a3-3b3b18afc417","slug":"singlet-oxygen","display_name":"Singlet molecular oxygen","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"2d98de70-03a6-504d-9fb2-2603668a358a","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-mixture-quenching-event","event_type":"biochemical_relationship","label":"The three pigments worked better together in this solution experiment.","description":"The equal-ratio meso-zeaxanthin, zeaxanthin and lutein mixture quenched more singlet oxygen than each individual pigment at the same total concentration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd84367-3f8e-5a3f-b952-a96174ea51fa","slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"},"role":"mixture_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a60c685-7502-5ece-9da2-2c1a2e3bcdf1","slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"},"role":"mixture_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"24cea833-2dbe-5746-be4d-06b3b7c6315c","slug":"meso-zeaxanthin","display_name":"meso-Zeaxanthin / (3R,3-prime-S)-zeaxanthin","entity_type_key":"small_molecule"},"role":"mixture_component","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9bd3f7b4-0e76-5586-9eef-16e0b30865be","slug":"macular-carotenoid-111-mixture","display_name":"Meso-zeaxanthin, zeaxanthin and lutein 1:1:1 assay mixture","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ac4a9e3e-1454-5802-82a3-3b3b18afc417","slug":"singlet-oxygen","display_name":"Singlet molecular oxygen","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lutein-research/20678467.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"aaf88f567941ed16adb101981a218960bbd8ae3cfe0919868de63462e7c7be01\", \"start_char\": 0, \"end_char\": 989, \"text_sha256\": \"aaf88f567941ed16adb101981a218960bbd8ae3cfe0919868de63462e7c7be01\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"EPR of donor eye tissue and solution quenching assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"White-light challenge and equal-total-concentration carotenoid comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Solution mixture effects do not establish an optimal oral ratio or clinical benefit; exogenous pigment addition differs from delivery in a living eye.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lutein research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Postmortem human tissue and cell-free systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The three pigments worked better together in this solution experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lutein-p20678467] Studies on the singlet oxygen scavenging mechanism of human macular pigment. (2010). https://pubmed.ncbi.nlm.nih.gov/20678467/ DOI: 10.1016/j.abb.2010.07.024","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Macula, RPE/choroid and carotenoid solutions","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b3f93d57-ebc4-5493-aba2-689ddcad17af","evidence_kind":"source_excerpt","locator":"Lines 229-240","start_line":229,"end_line":240,"excerpt":"### lutein-mixture-quenching\nThe equal-ratio meso-zeaxanthin, zeaxanthin and lutein mixture quenched more singlet oxygen than each individual pigment at the same total concentration.\nCondition category: normal\nnutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The three pigments worked better together in this solution experiment.\norganism: Postmortem human tissue and cell-free systems\ntissue_or_cell_type: Macula, RPE/choroid and carotenoid solutions\nexperimental_model: EPR of donor eye tissue and solution quenching assays\nlimitations: Solution mixture effects do not establish an optimal oral ratio or clinical benefit; exogenous pigment addition differs from delivery in a living eye.\nexposure: White-light challenge and equal-total-concentration carotenoid comparisons\nevidence_span: {\"source_cache\": \"artifacts/lutein-research/20678467.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"aaf88f567941ed16adb101981a218960bbd8ae3cfe0919868de63462e7c7be01\", \"start_char\": 0, \"end_char\": 989, \"text_sha256\": \"aaf88f567941ed16adb101981a218960bbd8ae3cfe0919868de63462e7c7be01\"}\n[lutein-p20678467] Studies on the singlet oxygen scavenging mechanism of human macular pigment. 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