{"id":"5481ceb0-2c63-5c47-8880-1917cbcc1f6f","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-intestinal-ph-transport","predicate":"undergoes","statement":"Caco-2 lipoic-acid transport was energy dependent, increased at acidic pH and was inhibited by tested monocarboxylates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"37819f0e-b70f-5c47-9962-e9a52567d09c","mechanism_event_label":"Absorption behavior depended on acidity and competing molecules in this cell model.","subject":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"1df5d45a-98c6-5266-92d3-ee4b28da1223","slug":"intestinal-monocarboxylate-sensitive-lipoate-transport","display_name":"Intestinal monocarboxylate-sensitive-lipoate transport","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"37819f0e-b70f-5c47-9962-e9a52567d09c","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-intestinal-ph-transport-event","event_type":"biochemical_relationship","label":"Absorption behavior depended on acidity and competing molecules in this cell model.","description":"Caco-2 lipoic-acid transport was energy dependent, increased at acidic pH and was inhibited by tested monocarboxylates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1df5d45a-98c6-5266-92d3-ee4b28da1223","slug":"intestinal-monocarboxylate-sensitive-lipoate-transport","display_name":"Intestinal monocarboxylate-sensitive-lipoate transport","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/17536819.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d777a0163a5cb3c8de0edf282cca050a720098f5ee7b0f4e22f9a0b3275c5b7c\", \"start_char\": 0, \"end_char\": 863, \"text_sha256\": \"d777a0163a5cb3c8de0edf282cca050a720098f5ee7b0f4e22f9a0b3275c5b7c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Caco-2 intestinal transport model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lipoic acid transport across pH and competing-monocarboxylate conditions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell model suggests proton-linked monocarboxylate transport but does not identify a specific SLC16 isoform or quantify whole-person absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Absorption behavior depended on acidity and competing molecules in this cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p17536819] Transepithelial transport of alpha-lipoic acid across human intestinal Caco-2 cell monolayers. (2007). https://pubmed.ncbi.nlm.nih.gov/17536819/ DOI: 10.1021/jf063624i","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelial monolayer","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"35319640-86eb-5096-ad38-eef6c490218f","evidence_kind":"source_excerpt","locator":"Lines 611-622","start_line":611,"end_line":622,"excerpt":"### ala-intestinal-ph-transport\nCaco-2 lipoic-acid transport was energy dependent, increased at acidic pH and was inhibited by tested monocarboxylates.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Absorption behavior depended on acidity and competing molecules in this cell model.\norganism: Human cell line\ntissue_or_cell_type: Intestinal epithelial monolayer\nexperimental_model: Caco-2 intestinal transport model\nlimitations: Cell model suggests proton-linked monocarboxylate transport but does not identify a specific SLC16 isoform or quantify whole-person absorption.\nexposure: Lipoic acid transport across pH and competing-monocarboxylate conditions\nevidence_span: {\"source_cache\": \"artifacts/ala-research/17536819.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d777a0163a5cb3c8de0edf282cca050a720098f5ee7b0f4e22f9a0b3275c5b7c\", \"start_char\": 0, \"end_char\": 863, \"text_sha256\": \"d777a0163a5cb3c8de0edf282cca050a720098f5ee7b0f4e22f9a0b3275c5b7c\"}\n[ala-p17536819] Transepithelial transport of alpha-lipoic acid across human intestinal Caco-2 cell monolayers. 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