{"id":"547323e8-426c-545c-bd10-917162e01c5f","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-histamine","predicate":"reduces_stimulated_release","statement":"SS-FA at 0.1-10 micrograms/mL reduced PMA/calcium-ionophore-induced histamine release from KU812 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"61123306-7519-5ed9-908d-ab4df483e35a","mechanism_event_label":"One preparation reduced triggered mediator release.","subject":{"id":"4402078e-d703-56fb-b9e4-37265c02cf8e","slug":"sludge-fulvic-2011","display_name":"Solubilized-sludge fulvic fraction, 2011 study","entity_type_key":"chemical_species"},"object":{"id":"847daefd-ce13-541a-b426-919975168a3d","slug":"histamine","display_name":"Histamine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"61123306-7519-5ed9-908d-ab4df483e35a","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-histamine-event","event_type":"observed_relationship","label":"One preparation reduced triggered mediator release.","description":"SS-FA at 0.1-10 micrograms/mL reduced PMA/calcium-ionophore-induced histamine release from KU812 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4402078e-d703-56fb-b9e4-37265c02cf8e","slug":"sludge-fulvic-2011","display_name":"Solubilized-sludge fulvic fraction, 2011 study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"847daefd-ce13-541a-b426-919975168a3d","slug":"histamine","display_name":"Histamine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human basophilic leukemia KU812 cells; 15-minute pretreatment, PMA 20 nM plus A23187 1 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Sludge-derived research material is not a dietary ingredient recommendation. This does not establish human allergy treatment or direct calcium chelation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"One preparation reduced triggered mediator release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21331654/ · DOI 10.1007/s10616-010-9333-6","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be24e668-f9f8-5440-92d6-31d8d826434c","evidence_kind":"source_excerpt","locator":"Lines 108-114","start_line":108,"end_line":114,"excerpt":"## fulvic-acid-histamine\nOne preparation reduced triggered mediator release.\nSS-FA at 0.1-10 micrograms/mL reduced PMA/calcium-ionophore-induced histamine release from KU812 cells.\nModel: Human basophilic leukemia KU812 cells; 15-minute pretreatment, PMA 20 nM plus A23187 1 micromolar.\nLimitations: Sludge-derived research material is not a dietary ingredient recommendation. This does not establish human allergy treatment or direct calcium chelation.\nEvidence access: Primary abstract\nMicroarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21331654/ · DOI 10.1007/s10616-010-9333-6","model_system":"Human basophilic leukemia KU812 cells; 15-minute pretreatment, PMA 20 nM plus A23187 1 micromolar.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}