{"id":"541cc8d0-efd1-5441-8af2-fd074b47dac4","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-glucose-rescue","predicate":"rescues","statement":"Adding 1 mM alanine or pyruvate rescued proliferation under 0.5 mM glucose in the tested human PDAC cultures, while 1 mM lactate did not reproduce that rescue.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"39304065-7fec-5da9-ad4d-0a8d3c01ebd2","mechanism_event_label":"Different carbon sources were not interchangeable in the nutrient-limited culture.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"6b99cdbb-354c-5dd6-810a-e4637744da58","slug":"human-pdac-low-glucose-growth","display_name":"Growth of human PDAC cells under low glucose","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"39304065-7fec-5da9-ad4d-0a8d3c01ebd2","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-glucose-rescue-event","event_type":"observed_relationship","label":"Different carbon sources were not interchangeable in the nutrient-limited culture.","description":"Adding 1 mM alanine or pyruvate rescued proliferation under 0.5 mM glucose in the tested human PDAC cultures, while 1 mM lactate did not reproduce that rescue.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b99cdbb-354c-5dd6-810a-e4637744da58","slug":"human-pdac-low-glucose-growth","display_name":"Growth of human PDAC cells under low glucose","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"8988T, Tu8902 and MiaPaCa2 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture rescue does not establish safe dietary restriction or supplementation strategies. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Different carbon sources were not interchangeable in the nutrient-limited culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"02b7d429-55b5-5845-bfa0-fab6d5ef041e","evidence_kind":"source_excerpt","locator":"Lines 168-174","start_line":168,"end_line":174,"excerpt":"## alanine-pdac-glucose-rescue\nDifferent carbon sources were not interchangeable in the nutrient-limited culture.\nAdding 1 mM alanine or pyruvate rescued proliferation under 0.5 mM glucose in the tested human PDAC cultures, while 1 mM lactate did not reproduce that rescue.\nModel: 8988T, Tu8902 and MiaPaCa2 cell experiments.\nLimitations: Culture rescue does not establish safe dietary restriction or supplementation strategies. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf\nEvidence access: Primary full text\nPancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","model_system":"8988T, Tu8902 and MiaPaCa2 cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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