{"id":"53efbed4-1b8d-522d-84a4-fd3d7c6a6562","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-glucuronide-tak1","predicate":"reduces_activation","statement":"Luteolin 7-O-glucuronide reduced TAK1-pathway activation in LPS-stimulated RAW264.7 macrophages.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b685a943-be7d-5649-a336-2a4a77c304e5","mechanism_event_label":"A conjugate has its own experimentally observed activity.","subject":{"id":"4afd87a9-44dd-5774-8348-3dc09cde3e2a","slug":"luteolin-7-o-glucuronide","display_name":"Luteolin 7-O-glucuronide","entity_type_key":"small_molecule"},"object":{"id":"e79a2bd1-a859-5be3-b421-1fc291ca4fee","slug":"mouse-map3k7","display_name":"Mouse TAK1 / Map3k7","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b685a943-be7d-5649-a336-2a4a77c304e5","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-glucuronide-tak1-event","event_type":"observed_relationship","label":"A conjugate has its own experimentally observed activity.","description":"Luteolin 7-O-glucuronide reduced TAK1-pathway activation in LPS-stimulated RAW264.7 macrophages.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4afd87a9-44dd-5774-8348-3dc09cde3e2a","slug":"luteolin-7-o-glucuronide","display_name":"Luteolin 7-O-glucuronide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e79a2bd1-a859-5be3-b421-1fc291ca4fee","slug":"mouse-map3k7","display_name":"Mouse TAK1 / Map3k7","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophages, 5–50 micromolar pretreatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reduced phosphorylation is not proof of direct kinase binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A conjugate has its own experimentally observed activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory and Anti-Oxidative Effects of luteolin-7-O-glucuronide in LPS-Stimulated Murine Macrophages through TAK1 Inhibition and Nrf2 Activation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32187984/ · DOI 10.3390/ijms21062007","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"58703295-9412-5069-b1a3-3951a24d2a93","evidence_kind":"source_excerpt","locator":"Lines 500-506","start_line":500,"end_line":506,"excerpt":"## luteolin-glucuronide-tak1\nA conjugate has its own experimentally observed activity.\nLuteolin 7-O-glucuronide reduced TAK1-pathway activation in LPS-stimulated RAW264.7 macrophages.\nModel: Mouse macrophages, 5–50 micromolar pretreatment.\nLimitations: Reduced phosphorylation is not proof of direct kinase binding.\nEvidence access: Primary full text\nAnti-Inflammatory and Anti-Oxidative Effects of luteolin-7-O-glucuronide in LPS-Stimulated Murine Macrophages through TAK1 Inhibition and Nrf2 Activation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32187984/ · DOI 10.3390/ijms21062007","model_system":"Mouse macrophages, 5–50 micromolar pretreatment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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