{"id":"53c77a0d-d8ea-59ee-a2a7-c4ca465014e4","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-egtuc-binding","predicate":"binds","statement":"The Streptococcus pneumoniae EgtUC domain recognized the betaine and thioimidazole portions of ergothioneine with high specificity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"60846c00-3c66-5ba8-ba51-c37813cca198","mechanism_event_label":"A binding component selects the cargo.","subject":{"id":"dc23d911-85db-57ff-9d63-346ff98dc1c6","slug":"streptococcus-pneumoniae-egtuc","display_name":"Streptococcus pneumoniae EgtU solute-binding domain EgtUC","entity_type_key":"protein"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"60846c00-3c66-5ba8-ba51-c37813cca198","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-egtuc-binding-event","event_type":"observed_relationship","label":"A binding component selects the cargo.","description":"The Streptococcus pneumoniae EgtUC domain recognized the betaine and thioimidazole portions of ergothioneine with high specificity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc23d911-85db-57ff-9d63-346ff98dc1c6","slug":"streptococcus-pneumoniae-egtuc","display_name":"Streptococcus pneumoniae EgtU solute-binding domain EgtUC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ligand-bound bacterial transporter-domain structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Substrate recognition is distinct from ATP-driven translocation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A binding component selects the cargo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36481738/ · DOI 10.1038/s41467-022-35277-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"57aec76f-16db-574c-b7be-7ac31b919c75","evidence_kind":"source_excerpt","locator":"Lines 240-246","start_line":240,"end_line":246,"excerpt":"## ergothioneine-egtuc-binding\nA binding component selects the cargo.\nThe Streptococcus pneumoniae EgtUC domain recognized the betaine and thioimidazole portions of ergothioneine with high specificity.\nModel: Ligand-bound bacterial transporter-domain structure.\nLimitations: Substrate recognition is distinct from ATP-driven translocation.\nEvidence access: Primary abstract\nDiscovery and structure of a widespread bacterial ABC transporter specific for ergothioneine. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36481738/ · DOI 10.1038/s41467-022-35277-3","model_system":"Ligand-bound bacterial transporter-domain structure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}