{"id":"53a84021-eb17-5603-8e5e-16bd6860fc9f","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:raw-hsp72","predicate":"increases_in_recorded_experiment","statement":"Thymulin increased HSP72 in the macrophage experiment.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9a229cee-35ea-54ed-9523-398eee116dc6","mechanism_event_label":"Thymulin increased HSP72 in the macrophage experiment.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"49e779d6-ef34-5ffb-aaff-7173c4eeb5b7","slug":"mouse-hsp72","display_name":"Mouse HSP72 assay pool","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"9a229cee-35ea-54ed-9523-398eee116dc6","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:raw-hsp72-event","event_type":"experimental_observation","label":"Thymulin increased HSP72 in the macrophage experiment.","description":"| RAW264.7 macrophages, unstimulated versus LPS-stimulated | In resting cells, thymulin increased proinflammatory cytokine secretion. In LPS-stimulated cells, it reduced SAPK/JNK activity but not NF-κB activity; peptide alone did not change the measured cytokine secretion. HSP72 increased. | Preserve the resting/stimulated contrast. Thymopentin was also studied and requires separate attribution. [2011 experiment](https://pubmed.ncbi.nlm.nih.gov/22148922/) |","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin exposure","sequence_order":0,"notes":""},{"entity":{"id":"49e779d6-ef34-5ffb-aaff-7173c4eeb5b7","slug":"mouse-hsp72","display_name":"Mouse HSP72 assay pool","entity_type_key":"protein_family"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin exposure\", \"comparator\": \"Study control\", \"endpoint\": \"Thymulin increased HSP72 in the macrophage experiment.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse RAW264.7 macrophages; protein readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. 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In LPS-stimulated cells, it reduced SAPK/JNK activity but not NF-κB activity; peptide alone did not change the measured cytokine secretion. HSP72 increased. | Preserve the resting/stimulated contrast. Thymopentin was also studied and requires separate attribution. [2011 experiment](https://pubmed.ncbi.nlm.nih.gov/22148922/) |","model_system":"Mouse RAW264.7 macrophages; protein readout.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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