{"id":"53836be8-f2a8-5958-bf2f-ae2f9ad081c0","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-calmodulin-calcium-complex","predicate":"binds","statement":"Calcium-loaded calmodulin forms the Ca2+/CaM regulatory complex resolved with a myosin-light-chain-kinase target peptide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2fde1b0d-394c-5c6d-9799-6a509b415b60","mechanism_event_label":"Calmodulin carries bound calcium while engaging a target protein.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"cf536e74-d4df-5b54-a78f-dd002fa5dd83","slug":"calmodulin","display_name":"Calmodulin","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2fde1b0d-394c-5c6d-9799-6a509b415b60","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-calmodulin-calcium-complex-event","event_type":"binding","label":"Calmodulin carries bound calcium while engaging a target protein.","description":"Calcium-loaded calmodulin forms the Ca2+/CaM regulatory complex resolved with a myosin-light-chain-kinase target peptide.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"regulatory_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf536e74-d4df-5b54-a78f-dd002fa5dd83","slug":"calmodulin","display_name":"Calmodulin","entity_type_key":"protein"},"role":"calcium_sensor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a79ecb55-2003-5544-9599-c2136624394a","slug":"calcium-calmodulin","display_name":"Calcium/calmodulin","entity_type_key":"protein_complex"},"role":"protein_complex","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bc9ed3a1-38d5-5bc3-b2a5-9f9f7633660b","slug":"cytosol","display_name":"Cytosol","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Cytosolic protein complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified calcium-bound calmodulin and a chicken smooth-muscle myosin light-chain kinase peptide; crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure of a peptide complex; not a measurement of dietary calcium effects or every calmodulin target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Purified vertebrate proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Calmodulin carries bound calcium while engaging a target protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-meador1992] Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex (1992). https://pubmed.ncbi.nlm.nih.gov/1519061/ DOI: 10.1126/science.1519061","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"binding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Smooth-muscle target-peptide system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"829774ad-a4b2-58ee-936c-97cf0ba1c040","evidence_kind":"source_excerpt","locator":"Lines 399-410","start_line":399,"end_line":410,"excerpt":"### ca-calmodulin-calcium-complex\nCalcium-loaded calmodulin forms the Ca2+/CaM regulatory complex resolved with a myosin-light-chain-kinase target peptide.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Calmodulin carries bound calcium while engaging a target protein.\norganism: Purified vertebrate proteins\ntissue_or_cell_type: Smooth-muscle target-peptide system\nexperimental_model: Purified calcium-bound calmodulin and a chicken smooth-muscle myosin light-chain kinase peptide; crystallography\nlimitations: Structure of a peptide complex; not a measurement of dietary calcium effects or every calmodulin target.\nresearch_relationship_category: binding\ncompartment_description: Cytosolic protein complex\n[ca-meador1992] Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex (1992). https://pubmed.ncbi.nlm.nih.gov/1519061/ DOI: 10.1126/science.1519061","model_system":"Purified calcium-bound calmodulin and a chicken smooth-muscle myosin light-chain kinase peptide; crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-meador1992] Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex (1992). https://pubmed.ncbi.nlm.nih.gov/1519061/ DOI: 10.1126/science.1519061","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}