{"id":"5370e91c-0de9-50c8-a033-482d3ebfe0ed","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-stress","predicate":"reverses_deprivation_induction","statement":"ATF4 protein increased after three hours of T-cell activation in dialyzed-serum medium and returned toward control with alanine supplementation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ed6e738-36f1-5e73-9fa1-34533b4e26d7","mechanism_event_label":"Amino-acid stress signaling responded when alanine was restored.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"7a0fa32a-a00f-5500-bce7-8e8d101a8e2b","slug":"mouse-atf4","display_name":"Mouse activating transcription factor 4 / Atf4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4ed6e738-36f1-5e73-9fa1-34533b4e26d7","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-stress-event","event_type":"observed_relationship","label":"Amino-acid stress signaling responded when alanine was restored.","description":"ATF4 protein increased after three hours of T-cell activation in dialyzed-serum medium and returned toward control with alanine supplementation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a0fa32a-a00f-5500-bce7-8e8d101a8e2b","slug":"mouse-atf4","display_name":"Mouse activating transcription factor 4 / Atf4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ATF4 measurement supports a stress response; direct GCN2 necessity was not established in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Amino-acid stress signaling responded when alanine was restored.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7861874a-2e6e-553a-9bd2-c549fc51f0bf","evidence_kind":"source_excerpt","locator":"Lines 112-118","start_line":112,"end_line":118,"excerpt":"## alanine-t-cell-stress\nAmino-acid stress signaling responded when alanine was restored.\nATF4 protein increased after three hours of T-cell activation in dialyzed-serum medium and returned toward control with alanine supplementation.\nModel: Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.\nLimitations: ATF4 measurement supports a stress response; direct GCN2 necessity was not established in this experiment.\nEvidence access: Primary full text\nT Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","model_system":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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