{"id":"5358ce19-f989-5b74-bb07-a482440dfc0a","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-pcbd-transcription","predicate":"coactivates_hnf1b_control_of","statement":"Overexpressed wild-type PCBD1 bound HNF1B and increased FXYD2 promoter activity in a human kidney cell line.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"bb6f40fd-5f2b-5c5f-8efb-75af4913e1fc","mechanism_event_label":"A protein used in phenylalanine metabolism also helps control a kidney transport regulator.","subject":{"id":"a69cefc1-302d-5b87-8499-ecbe52b4059e","slug":"pcbd1","display_name":"Human pterin-4-alpha-carbinolamine dehydratase / PCBD1","entity_type_key":"protein"},"object":{"id":"56bd9a20-0c4b-508c-89a7-1195084827fd","slug":"fxyd2","display_name":"Human sodium/potassium ATPase regulatory subunit / FXYD2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bb6f40fd-5f2b-5c5f-8efb-75af4913e1fc","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-pcbd-transcription-event","event_type":"observed_relationship","label":"A protein used in phenylalanine metabolism also helps control a kidney transport regulator.","description":"Overexpressed wild-type PCBD1 bound HNF1B and increased FXYD2 promoter activity in a human kidney cell line.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a69cefc1-302d-5b87-8499-ecbe52b4059e","slug":"pcbd1","display_name":"Human pterin-4-alpha-carbinolamine dehydratase / PCBD1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"56bd9a20-0c4b-508c-89a7-1195084827fd","slug":"fxyd2","display_name":"Human sodium/potassium ATPase regulatory subunit / FXYD2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da9042c2-976d-5bbc-af38-979e07906c49","slug":"hnf1b","display_name":"Human hepatocyte nuclear factor 1 beta / HNF1B","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"66e1e9be-15e3-5431-9f49-b07ae3a99b5a","slug":"renal-magnesium-reabsorption","display_name":"Renal magnesium reabsorption","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human kidney-cell overexpression and promoter assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Promoter activity is not a direct measurement of magnesium flux or proof that phenylalanine intake regulates magnesium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A protein used in phenylalanine metabolism also helps control a kidney transport regulator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24204001/ · DOI 10.1681/ASN.2013040337","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ea8731f6-90ac-597f-82f3-0ef69c2d455f","evidence_kind":"source_excerpt","locator":"Lines 78-84","start_line":78,"end_line":84,"excerpt":"## l-phenylalanine-pcbd-transcription\nA protein used in phenylalanine metabolism also helps control a kidney transport regulator.\nOverexpressed wild-type PCBD1 bound HNF1B and increased FXYD2 promoter activity in a human kidney cell line.\nModel: Human kidney-cell overexpression and promoter assay.\nLimitations: Promoter activity is not a direct measurement of magnesium flux or proof that phenylalanine intake regulates magnesium.\nEvidence access: Primary abstract\nMutations in PCBD1 cause hypomagnesemia and renal magnesium wasting. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24204001/ · DOI 10.1681/ASN.2013040337","model_system":"Human kidney-cell overexpression and promoter assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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