{"id":"533e43bc-2301-5eb7-b5ba-9ff6f11c6fde","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-growth","predicate":"rescues","statement":"Alanine add-back restored growth and proliferation impaired by dialyzed-serum culture during initial activation of mouse naive T cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2ef192d5-6ce2-5ef3-9e0f-9a0502182f2e","mechanism_event_label":"A cell may be able to make alanine yet fail to make it fast enough when demand rises.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"fc5fb410-f62b-57e1-9eae-b94d1532db04","slug":"mouse-t-cell-activation-growth","display_name":"Growth and proliferation during mouse T-cell activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2ef192d5-6ce2-5ef3-9e0f-9a0502182f2e","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-growth-event","event_type":"observed_relationship","label":"A cell may be able to make alanine yet fail to make it fast enough when demand rises.","description":"Alanine add-back restored growth and proliferation impaired by dialyzed-serum culture during initial activation of mouse naive T cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc5fb410-f62b-57e1-9eae-b94d1532db04","slug":"mouse-t-cell-activation-growth","display_name":"Growth and proliferation during mouse T-cell activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dialysis removes multiple small nutrients; alanine rescue supports its contribution. This does not establish a human dietary deficiency or immune-boosting dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A cell may be able to make alanine yet fail to make it fast enough when demand rises.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e6971746-f808-560c-bb71-ae30b818a923","evidence_kind":"source_excerpt","locator":"Lines 80-86","start_line":80,"end_line":86,"excerpt":"## alanine-t-cell-growth\nA cell may be able to make alanine yet fail to make it fast enough when demand rises.\nAlanine add-back restored growth and proliferation impaired by dialyzed-serum culture during initial activation of mouse naive T cells.\nModel: Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.\nLimitations: Dialysis removes multiple small nutrients; alanine rescue supports its contribution. This does not establish a human dietary deficiency or immune-boosting dose.\nEvidence access: Primary full text\nT Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","model_system":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}