{"id":"5269eb89-4ff7-5a4e-ab99-96fdca2b7849","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ace2-transporter","predicate":"loss_reduces","statement":"Ace2 loss removed intestinal B0AT1 expression in mice; kidney B0AT1 regulation differed and depended on collectrin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a53f2ef1-be63-502e-8833-528ae3ecf441","mechanism_event_label":"The same amino-acid transporter needs different support in intestine and kidney.","subject":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"object":{"id":"b987295b-26b7-5c9a-a033-c5e6ce272186","slug":"mouse-slc6a19","display_name":"Mouse B0AT1 / Slc6a19","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a53f2ef1-be63-502e-8833-528ae3ecf441","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ace2-transporter-event","event_type":"observed_relationship","label":"The same amino-acid transporter needs different support in intestine and kidney.","description":"Ace2 loss removed intestinal B0AT1 expression in mice; kidney B0AT1 regulation differed and depended on collectrin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b987295b-26b7-5c9a-a033-c5e6ce272186","slug":"mouse-slc6a19","display_name":"Mouse B0AT1 / Slc6a19","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c855746f-55b6-5e99-9938-1043c74f7d84","slug":"mouse-intestinal-tryptophan-uptake","display_name":"Mouse intestinal tryptophan uptake","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, partner constructs and mouse tissue results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse tissue expression and transporter-partner experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Separate oocyte experiments combine human B0AT1 with mouse ACE2 or human collectrin; they are not an all-human ACE2 reconstruction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same amino-acid transporter needs different support in intestine and kidney.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19185582/ · DOI 10.1053/j.gastro.2008.10.055","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"abff16e6-5670-5c23-980e-4ca34b091250","evidence_kind":"source_excerpt","locator":"Lines 66-72","start_line":66,"end_line":72,"excerpt":"## tryptophan-ace2-transporter\nThe same amino-acid transporter needs different support in intestine and kidney.\nAce2 loss removed intestinal B0AT1 expression in mice; kidney B0AT1 regulation differed and depended on collectrin.\nModel: Mouse tissue expression and transporter-partner experiments.\nLimitations: Separate oocyte experiments combine human B0AT1 with mouse ACE2 or human collectrin; they are not an all-human ACE2 reconstruction.\nEvidence access: Primary full text, partner constructs and mouse tissue results\nTissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19185582/ · DOI 10.1053/j.gastro.2008.10.055","model_system":"Mouse tissue expression and transporter-partner experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}