{"id":"5259995b-7382-5e92-83aa-ee551421b4b7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mtfmt-folate-formylation","predicate":"formylates","statement":"MTFMT uses mitochondrial 10-formyl-THF to generate formylmethionyl-tRNA needed for translation initiation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"075697df-a8ed-565b-841d-2bde2dfdde21","mechanism_event_label":"Folate supplies the small chemical tag used to start mitochondrial proteins.","subject":{"id":"f6b7e980-fc6b-5c64-aa8e-9483d3d96029","slug":"mtfmt","display_name":"Human mitochondrial methionyl-tRNA formyltransferase / MTFMT","entity_type_key":"protein"},"object":{"id":"2a8e2612-d381-52ab-9cd6-81247da34019","slug":"mitochondrial-methionyl-trna","display_name":"Mitochondrial methionyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"075697df-a8ed-565b-841d-2bde2dfdde21","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mtfmt-folate-formylation-event","event_type":"biochemical_relationship","label":"Folate supplies the small chemical tag used to start mitochondrial proteins.","description":"MTFMT uses mitochondrial 10-formyl-THF to generate formylmethionyl-tRNA needed for translation initiation.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"ec73b364-a2ea-5c4a-bcea-a54aeebf57d1","slug":"10-formyltetrahydrofolate","display_name":"10-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"formyl donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5ef1fbd8-381c-5980-95bc-b500a134092f","slug":"mitochondrial-formylmethionyl-trna","display_name":"Mitochondrial formylmethionyl-tRNA","entity_type_key":"rna"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"folate coproduct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f6b7e980-fc6b-5c64-aa8e-9483d3d96029","slug":"mtfmt","display_name":"Human mitochondrial methionyl-tRNA formyltransferase / MTFMT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2a8e2612-d381-52ab-9cd6-81247da34019","slug":"mitochondrial-methionyl-trna","display_name":"Mitochondrial methionyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Genetic and tRNA-formylation experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not imply that plasma folate directly reports mitochondrial folate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Folate supplies the small chemical tag used to start mitochondrial proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[minton-2018] Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs (2018). https://pubmed.ncbi.nlm.nih.gov/29452640/ DOI: 10.1016/j.molcel.2018.01.024","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Jurkat cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b7c3d494-bc85-5fb0-a893-ed1887a07fac","evidence_kind":"source_excerpt","locator":"Lines 1133-1143","start_line":1133,"end_line":1143,"excerpt":"### mtfmt-folate-formylation\nMTFMT uses mitochondrial 10-formyl-THF to generate formylmethionyl-tRNA needed for translation initiation.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Folate supplies the small chemical tag used to start mitochondrial proteins.\norganism: Homo sapiens\ntissue_or_cell_type: Jurkat cells\nexperimental_model: Genetic and tRNA-formylation experiments\nlimitations: Does not imply that plasma folate directly reports mitochondrial folate.\nexposure: Assay conditions described in the linked primary study.\n[minton-2018] Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs (2018). https://pubmed.ncbi.nlm.nih.gov/29452640/ DOI: 10.1016/j.molcel.2018.01.024","model_system":"Genetic and tRNA-formylation experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [minton-2018] Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs (2018). https://pubmed.ncbi.nlm.nih.gov/29452640/ DOI: 10.1016/j.molcel.2018.01.024","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}