{"id":"524895be-8602-5e1c-be71-5eb34a3d70ec","stable_key":"6660bba2-4fa0-590a-bf52-9803a24bea9f:noradrenergic-lesion","predicate":"no_detected_change_in_recorded_experiment","statement":"DSP-4 removal of forebrain noradrenergic projections did not eliminate modafinil wake promotion, while an adrenergic antagonist still suppressed it.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"582bd3a3-5833-5e75-926d-acdab1bbd145","mechanism_event_label":"DSP-4 removal of forebrain noradrenergic projections did not eliminate modafinil wake promotion, while an adrenergic antagonist still suppressed it.","subject":{"id":"4d006e1d-52ba-5af1-afb0-2b34fbd52333","slug":"mouse-noradrenergic-projections","display_name":"Mouse forebrain noradrenergic projections","entity_type_key":"cellular_process"},"object":{"id":"2783faf9-3c82-5360-8540-e30f0f7310a1","slug":"mouse-wake-duration","display_name":"Mouse wake duration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"582bd3a3-5833-5e75-926d-acdab1bbd145","stable_key":"6660bba2-4fa0-590a-bf52-9803a24bea9f:noradrenergic-lesion-event","event_type":"experimental_observation","label":"DSP-4 removal of forebrain noradrenergic projections did not eliminate modafinil wake promotion, while an adrenergic antagonist still suppressed it.","description":"In mice, removing forebrain noradrenergic projections with DSP-4 did not eliminate wake promotion, whereas an adrenergic antagonist still suppressed it. The interpretation involved dopamine–adrenergic interactions; the result prevents treating an intact locus-coeruleus projection as an obligatory relay. [2005 intervention study](https://pubmed.ncbi.nlm.nih.gov/15857707/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4d006e1d-52ba-5af1-afb0-2b34fbd52333","slug":"mouse-noradrenergic-projections","display_name":"Mouse forebrain noradrenergic projections","entity_type_key":"cellular_process"},"role":"tested factor","stoichiometry":null,"state_label":"Noradrenergic projection lesion plus modafinil","sequence_order":0,"notes":""},{"entity":{"id":"2783faf9-3c82-5360-8540-e30f0f7310a1","slug":"mouse-wake-duration","display_name":"Mouse wake duration","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"624e6a4b-f96d-5d12-8206-30d1e1715c26","slug":"modafinil","display_name":"Modafinil","entity_type_key":"drug"},"role":"drug challenge","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"34ab29ee-eb2b-5e51-9d25-0c1f8d5b97d9","slug":"norepinephrine","display_name":"Norepinephrine","entity_type_key":"small_molecule"},"role":"transmitter","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract checked; full methods and figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Noradrenergic projection lesion plus modafinil\", \"comparator\": \"Modafinil without lesion\", \"endpoint\": \"DSP-4 removal of forebrain noradrenergic projections did not eliminate modafinil wake promotion, while an adrenergic antagonist still suppressed it.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse pharmacological lesion experiment; adrenergic involvement does not require a simple obligatory projection relay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"DSP-4 removal of forebrain noradrenergic projections did not eliminate modafinil wake promotion, while an adrenergic antagonist still suppressed it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Dopaminergic-adrenergic interactions in the wake promoting mechanism of modafinil. | 2005 | DOI 10.1016/j.neuroscience.2005.02.003 | PMID 15857707 | https://pubmed.ncbi.nlm.nih.gov/15857707/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 87-87; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5d17bead-2724-5bac-9ba7-35b5067cb46d","evidence_kind":"source_excerpt","locator":"Lines 87-87","start_line":87,"end_line":87,"excerpt":"In mice, removing forebrain noradrenergic projections with DSP-4 did not eliminate wake promotion, whereas an adrenergic antagonist still suppressed it. 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