{"id":"51d1d9a5-8d98-5a08-8e2f-fef066a6298c","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-bovine-dhfr","predicate":"inhibits","statement":"EGCG inhibited bovine DHFR with slow tight binding, Ki 0.109 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"683acf4c-0f01-56c7-bd9b-f94cc466a5c3","mechanism_event_label":"EGCG inhibited a folate-processing enzyme in a purified animal preparation.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"c011ba41-0bc8-5f40-abe2-d10d8de823f3","slug":"bovine-dhfr","display_name":"Bovine dihydrofolate reductase / DHFR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"683acf4c-0f01-56c7-bd9b-f94cc466a5c3","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-bovine-dhfr-event","event_type":"observed_relationship","label":"EGCG inhibited a folate-processing enzyme in a purified animal preparation.","description":"EGCG inhibited bovine DHFR with slow tight binding, Ki 0.109 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c011ba41-0bc8-5f40-abe2-d10d8de823f3","slug":"bovine-dhfr","display_name":"Bovine dihydrofolate reductase / DHFR","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not relabel the purified enzymes as human DHFR or infer that normal tea intake causes folate deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"EGCG inhibited a folate-processing enzyme in a purified animal preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The antifolate activity of tea catechins. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781612/ · DOI 10.1158/0008-5472.can-04-3469","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"12c337f0-4f88-50a0-96d7-71fb1907a7b5","evidence_kind":"source_excerpt","locator":"Lines 124-130","start_line":124,"end_line":130,"excerpt":"## egcg-bovine-dhfr\nEGCG inhibited a folate-processing enzyme in a purified animal preparation.\nEGCG inhibited bovine DHFR with slow tight binding, Ki 0.109 micromolar.\nModel: Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.\nLimitations: Do not relabel the purified enzymes as human DHFR or infer that normal tea intake causes folate deficiency.\nEvidence access: primary abstract.\nThe antifolate activity of tea catechins. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781612/ · DOI 10.1158/0008-5472.can-04-3469","model_system":"Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}