{"id":"51ce17f0-1938-59c7-8496-df7107074f3a","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-zebrafish-oxidation","predicate":"knockout_increases","statement":"ETT-knockout zebrafish had 3.8-fold higher skin 8-oxoguanine; lipid-oxidation differences appeared after lead or copper stress.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c9401aed-53e6-530b-8aba-52cb1c7fb669","mechanism_event_label":"Loss of protection can become clearer under stress.","subject":{"id":"fdf8a38f-1a41-5993-b6fd-2239cba9406b","slug":"zebrafish-slc22a4","display_name":"Zebrafish ergothioneine transporter ETT","entity_type_key":"protein"},"object":{"id":"4b5cdeba-d398-533a-95e8-758de58de250","slug":"zebrafish-skin-8-oxoguanine","display_name":"Zebrafish skin 8-oxoguanine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c9401aed-53e6-530b-8aba-52cb1c7fb669","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-zebrafish-oxidation-event","event_type":"observed_relationship","label":"Loss of protection can become clearer under stress.","description":"ETT-knockout zebrafish had 3.8-fold higher skin 8-oxoguanine; lipid-oxidation differences appeared after lead or copper stress.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fdf8a38f-1a41-5993-b6fd-2239cba9406b","slug":"zebrafish-slc22a4","display_name":"Zebrafish ergothioneine transporter ETT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4b5cdeba-d398-533a-95e8-758de58de250","slug":"zebrafish-skin-8-oxoguanine","display_name":"Zebrafish skin 8-oxoguanine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Whole-fish and skin analyses; basal versus metal-stressed conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A stress-dependent phenotype does not identify one exclusive antioxidant reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Loss of protection can become clearer under stress.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Knockout of the ergothioneine transporter ETT in zebrafish results in increased 8-oxoguanine levels. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25746775/ · DOI 10.1016/j.freeradbiomed.2015.02.026","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"dc99feff-6cdc-5df0-85fc-33244f371ae4","evidence_kind":"source_excerpt","locator":"Lines 104-110","start_line":104,"end_line":110,"excerpt":"## ergothioneine-zebrafish-oxidation\nLoss of protection can become clearer under stress.\nETT-knockout zebrafish had 3.8-fold higher skin 8-oxoguanine; lipid-oxidation differences appeared after lead or copper stress.\nModel: Whole-fish and skin analyses; basal versus metal-stressed conditions.\nLimitations: A stress-dependent phenotype does not identify one exclusive antioxidant reaction.\nEvidence access: Primary abstract\nKnockout of the ergothioneine transporter ETT in zebrafish results in increased 8-oxoguanine levels. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25746775/ · DOI 10.1016/j.freeradbiomed.2015.02.026","model_system":"Whole-fish and skin analyses; basal versus metal-stressed conditions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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