{"id":"519612f3-18f0-5005-80df-e2df5b30bcf6","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-intestinal-knockout-biotin","predicate":"abolishes_carrier_mediated_uptake","statement":"Intestine-specific Slc5a6 deletion completely inhibited the measured carrier-mediated intestinal biotin uptake component relative to littermate controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fa9759df-fb4a-555f-84af-3f8af87926ff","mechanism_event_label":"Removing the shared vitamin transporter eliminated active intestinal biotin uptake in these mice.","subject":{"id":"ae553842-46ff-5783-83af-f3b334c47e8d","slug":"mouse-intestinal-slc5a6-null","display_name":"Intestine-specific Slc5a6-null mouse genotype","entity_type_key":"gene"},"object":{"id":"606e290e-05bc-5bce-80c5-0a0a444c462e","slug":"mouse-intestinal-biotin-uptake","display_name":"Mouse intestinal carrier-mediated biotin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fa9759df-fb4a-555f-84af-3f8af87926ff","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-intestinal-knockout-biotin-event","event_type":"biochemical_relationship","label":"Removing the shared vitamin transporter eliminated active intestinal biotin uptake in these mice.","description":"Intestine-specific Slc5a6 deletion completely inhibited the measured carrier-mediated intestinal biotin uptake component relative to littermate controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0cfc7721-a597-5e4b-9d7a-012fd8bc12fb","slug":"mouse-slc5a6","display_name":"Mouse sodium-dependent multivitamin transporter / Slc5a6","entity_type_key":"protein"},"role":"deleted_transport_machinery","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"measured_transport_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"other_shared_transport_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":"Not the directly measured substrate in the abstract result."},{"entity":{"id":"ae553842-46ff-5783-83af-f3b334c47e8d","slug":"mouse-intestinal-slc5a6-null","display_name":"Intestine-specific Slc5a6-null mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"606e290e-05bc-5bce-80c5-0a0a444c462e","slug":"mouse-intestinal-biotin-uptake","display_name":"Mouse intestinal carrier-mediated biotin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Intestine-specific Slc5a6 conditional knockout mice compared with sex-matched littermates; in vivo and in vitro uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cre/lox-mediated intestinal Slc5a6 deletion; in vivo and in vitro biotin transport assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is shared transporter machinery impairment, not a dietary B5 depletion experiment. The abstract directly reports biotin uptake; no numerical pantothenate or lipoate absorption deficit is inferred. Passive uptake outside the measured carrier component is not excluded.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing the shared vitamin transporter eliminated active intestinal biotin uptake in these mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-intestinal-knockout2013] Conditional knockout of the Slc5a6 gene in mouse intestine impairs biotin absorption. (2013). https://pubmed.ncbi.nlm.nih.gov/23104561/ DOI: 10.1152/ajpgi.00379.2012","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1bee8688-5bdc-574f-98c5-0b1c2fc0640e","evidence_kind":"source_excerpt","locator":"Lines 314-325","start_line":314,"end_line":325,"excerpt":"### b5-trans-intestinal-knockout-biotin\nIntestine-specific Slc5a6 deletion completely inhibited the measured carrier-mediated intestinal biotin uptake component relative to littermate controls.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing the shared vitamin transporter eliminated active intestinal biotin uptake in these mice.\norganism: Mus musculus\ntissue_or_cell_type: Intestinal epithelium\nexperimental_model: Intestine-specific Slc5a6 conditional knockout mice compared with sex-matched littermates; in vivo and in vitro uptake\nlimitations: This is shared transporter machinery impairment, not a dietary B5 depletion experiment. The abstract directly reports biotin uptake; no numerical pantothenate or lipoate absorption deficit is inferred. Passive uptake outside the measured carrier component is not excluded.\nexposure: Cre/lox-mediated intestinal Slc5a6 deletion; in vivo and in vitro biotin transport assays.\ncross_nutrient: true\n[b5-trans-intestinal-knockout2013] Conditional knockout of the Slc5a6 gene in mouse intestine impairs biotin absorption. (2013). https://pubmed.ncbi.nlm.nih.gov/23104561/ DOI: 10.1152/ajpgi.00379.2012","model_system":"Intestine-specific Slc5a6 conditional knockout mice compared with sex-matched littermates; in vivo and in vitro uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-intestinal-knockout2013] Conditional knockout of the Slc5a6 gene in mouse intestine impairs biotin absorption. (2013). https://pubmed.ncbi.nlm.nih.gov/23104561/ DOI: 10.1152/ajpgi.00379.2012","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}