{"id":"512fa63d-1baf-5dd2-8828-4641a461ccaa","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-pank4-phosphatase","predicate":"dephosphorylates","statement":"Immunopurified full-length human PANK4 hydrolyzed phosphate from 4′-phosphopantetheine; D623A or D659A substitutions abolished the phosphatase activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fdf711ab-6bc9-5f95-b818-f5a2d1f85bcf","mechanism_event_label":"PANK4 can remove a phosphate from a CoA-building intermediate.","subject":{"id":"19aa4674-db27-50f2-a232-44e427da4a5a","slug":"pank4","display_name":"Human pantothenate kinase 4 / PANK4","entity_type_key":"protein"},"object":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fdf711ab-6bc9-5f95-b818-f5a2d1f85bcf","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-pank4-phosphatase-event","event_type":"biochemical_relationship","label":"PANK4 can remove a phosphate from a CoA-building intermediate.","description":"Immunopurified full-length human PANK4 hydrolyzed phosphate from 4′-phosphopantetheine; D623A or D659A substitutions abolished the phosphatase activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a0138933-a2ed-58bb-b867-01aaf4fa7f0b","slug":"pantetheine","display_name":"Pantetheine","entity_type_key":"small_molecule"},"role":"dephosphorylated_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19aa4674-db27-50f2-a232-44e427da4a5a","slug":"pank4","display_name":"Human pantothenate kinase 4 / PANK4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cultured-cell metabolomics and isotope tracing, PANK4 editing/re-expression and immunopurified human PANK4 phosphatase assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Primary Fig. 4 biochemical assays with immunopurified Flag-PANK4, wild type versus D623A or D659A; substrate concentration not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct phosphatase assay. Divalent-metal participation was reported, but a particular nutritional mineral requirement is not assigned from these experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PANK4 can remove a phosphate from a CoA-building intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-bio-dibble2022] PI3K drives the de novo synthesis of coenzyme A from vitamin B5. (2022). https://pubmed.ncbi.nlm.nih.gov/35896750/ DOI: 10.1038/s41586-022-04984-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bad3ef4a-2276-5f40-b5ac-32824a9c188c","evidence_kind":"source_excerpt","locator":"Lines 665-676","start_line":665,"end_line":676,"excerpt":"### b5-bio-pank4-phosphatase\nImmunopurified full-length human PANK4 hydrolyzed phosphate from 4′-phosphopantetheine; D623A or D659A substitutions abolished the phosphatase activity.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PANK4 can remove a phosphate from a CoA-building intermediate.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Human cultured-cell metabolomics and isotope tracing, PANK4 editing/re-expression and immunopurified human PANK4 phosphatase assays\nlimitations: Direct phosphatase assay. Divalent-metal participation was reported, but a particular nutritional mineral requirement is not assigned from these experiments.\nexposure: Primary Fig. 4 biochemical assays with immunopurified Flag-PANK4, wild type versus D623A or D659A; substrate concentration not extracted.\ncross_nutrient: false\n[b5-bio-dibble2022] PI3K drives the de novo synthesis of coenzyme A from vitamin B5. (2022). https://pubmed.ncbi.nlm.nih.gov/35896750/ DOI: 10.1038/s41586-022-04984-8","model_system":"Human cultured-cell metabolomics and isotope tracing, PANK4 editing/re-expression and immunopurified human PANK4 phosphatase assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-bio-dibble2022] PI3K drives the de novo synthesis of coenzyme A from vitamin B5. (2022). https://pubmed.ncbi.nlm.nih.gov/35896750/ DOI: 10.1038/s41586-022-04984-8","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}