{"id":"5120aa1f-3d01-50e7-a34d-fb9476e5de47","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ruminococcal-tryptamine","predicate":"decarboxylates_to","statement":"Purified R. gnavus RUMGNA_01526 robustly decarboxylated tryptophan despite only 26% sequence identity to the C. sporogenes enzyme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a4ae0814-693c-551c-b220-9d80ea684881","mechanism_event_label":"Different microbes can reach the same metabolite with distinct proteins.","subject":{"id":"1ecf1dde-f6df-5248-8b86-85c6dcd101ac","slug":"r-gnavus-rumgna-01526","display_name":"Ruminococcus gnavus RUMGNA_01526 tryptophan decarboxylase","entity_type_key":"protein"},"object":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a4ae0814-693c-551c-b220-9d80ea684881","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ruminococcal-tryptamine-event","event_type":"observed_relationship","label":"Different microbes can reach the same metabolite with distinct proteins.","description":"Purified R. gnavus RUMGNA_01526 robustly decarboxylated tryptophan despite only 26% sequence identity to the C. sporogenes enzyme.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1ecf1dde-f6df-5248-8b86-85c6dcd101ac","slug":"r-gnavus-rumgna-01526","display_name":"Ruminococcus gnavus RUMGNA_01526 tryptophan decarboxylase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, enzyme screening and purification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enzyme presence and activity are not universal across human gut communities.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Different microbes can reach the same metabolite with distinct proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery and characterization of gut microbiota decarboxylases that can produce the neurotransmitter tryptamine. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25263219/ · DOI 10.1016/j.chom.2014.09.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dd1ddcd5-1dc3-5bee-a61d-b19d842ec5a4","evidence_kind":"source_excerpt","locator":"Lines 554-560","start_line":554,"end_line":560,"excerpt":"## tryptophan-ruminococcal-tryptamine\nDifferent microbes can reach the same metabolite with distinct proteins.\nPurified R. gnavus RUMGNA_01526 robustly decarboxylated tryptophan despite only 26% sequence identity to the C. sporogenes enzyme.\nModel: Purified bacterial enzyme assays.\nLimitations: Enzyme presence and activity are not universal across human gut communities.\nEvidence access: Primary full text, enzyme screening and purification\nDiscovery and characterization of gut microbiota decarboxylases that can produce the neurotransmitter tryptamine. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25263219/ · DOI 10.1016/j.chom.2014.09.001","model_system":"Purified bacterial enzyme assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}