{"id":"50f22dca-8922-571b-92b1-835702c9d83c","stable_key":"research:jurkat-selenop-use-persists-without-scly","predicate":"dispensable-for","statement":"Jurkat cells retained efficient SELENOP utilization without SCLY.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"context_dependent","is_public":true,"mechanism_event_id":"240dd4aa-a953-52e2-a880-77b3002127df","mechanism_event_label":"This cell line used a SCLY-independent recovery route.","subject":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"object":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"240dd4aa-a953-52e2-a880-77b3002127df","stable_key":"research:jurkat-selenop-use-persists-without-scly","event_type":"genetic-perturbation","label":"This cell line used a SCLY-independent recovery route.","description":"Jurkat cells retained efficient SELENOP utilization without SCLY.","status":"active","compartment":null,"participants":[{"entity":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"absent","sequence_order":0,"notes":""},{"entity":{"id":"c7fbd153-6444-50b2-a8be-6936e352a11c","slug":"selenop","display_name":"SELENOP","entity_type_key":"protein"},"role":"selenium-source","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"role":"readout","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"Jurkat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SCLY disruption","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Alternative recovery chemistry is unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6f744886-8331-5217-8821-da23157d0071","evidence_kind":"curated_literature_summary","locator":"lines 582-592","start_line":582,"end_line":592,"excerpt":"## jurkat-selenop-use-persists-without-scly\n\nThis cell line used a SCLY-independent recovery route.\n\nJurkat cells retained efficient SELENOP utilization without SCLY.\n\nOrganism: human\nCell type: Jurkat\nExperimental model: SCLY disruption\nLimitations: Alternative recovery chemistry is unresolved.\nPrimary reference: [An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase](https://pubmed.ncbi.nlm.nih.gov/37406814/)","model_system":"SCLY disruption","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"selenium-recycling","plain_language":"This cell line used a SCLY-independent recovery route.","evidence_scope":"direct_experimental","papers":[{"key":"mizuno-2023-lrp8-routing","title":"An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase","url":"https://pubmed.ncbi.nlm.nih.gov/37406814/","doi":"10.1016/j.jbc.2023.105009","year":2023,"model":"Human Jurkat and RD cells","summary":"Receptor variants and vesicle processing differ between cell lines; SCLY dependence is not universal."}]}}