{"id":"50714ead-6530-56a7-9e2a-f4cfa12020ae","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-mcc-complex","predicate":"assembles_with","statement":"Active recombinant human MCC contained alpha and beta subunits at a one-to-one ratio.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bcea8e79-0ad6-5cf1-bb8c-a606da641a12","mechanism_event_label":"MCC needs both subunits; biotin cannot replace a missing protein partner.","subject":{"id":"49c0fac5-6d59-5fe3-afda-89cad66c1324","slug":"mccc1","display_name":"Human methylcrotonyl-CoA carboxylase alpha subunit / MCCC1","entity_type_key":"protein"},"object":{"id":"c076f509-3f61-52b3-a301-63c3bbfa9b7d","slug":"mccc2","display_name":"Human methylcrotonyl-CoA carboxylase beta subunit / MCCC2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bcea8e79-0ad6-5cf1-bb8c-a606da641a12","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-mcc-complex-event","event_type":"biochemical_relationship","label":"MCC needs both subunits; biotin cannot replace a missing protein partner.","description":"Active recombinant human MCC contained alpha and beta subunits at a one-to-one ratio.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"871496e9-19bf-5fd0-a2a2-82d1b0bff8b4","slug":"human-mcc","display_name":"Human 3-methylcrotonyl-CoA carboxylase complex","entity_type_key":"protein_complex"},"role":"assembled enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49c0fac5-6d59-5fe3-afda-89cad66c1324","slug":"mccc1","display_name":"Human methylcrotonyl-CoA carboxylase alpha subunit / MCCC1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c076f509-3f61-52b3-a301-63c3bbfa9b7d","slug":"mccc2","display_name":"Human methylcrotonyl-CoA carboxylase beta subunit / MCCC2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/17360195.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3ae046490d9657d005d5c39efb1f7439bc015d0cb2190834e3e868de70eaf985\", \"start_char\": 0, \"end_char\": 1067, \"text_sha256\": \"3ae046490d9657d005d5c39efb1f7439bc015d0cb2190834e3e868de70eaf985\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human MCC produced by baculovirus expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ATP and methylcrotonyl-CoA kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recombinant-enzyme kinetics do not determine human dietary requirements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MCC needs both subunits; biotin cannot replace a missing protein partner.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p17360195] Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). (2007). https://pubmed.ncbi.nlm.nih.gov/17360195/ DOI: 10.1016/j.pep.2007.01.012","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human MCC complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b6e551b9-9786-5cd7-a518-9ccab547df2b","evidence_kind":"source_excerpt","locator":"Lines 767-778","start_line":767,"end_line":778,"excerpt":"### b7-mcc-complex\nActive recombinant human MCC contained alpha and beta subunits at a one-to-one ratio.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: MCC needs both subunits; biotin cannot replace a missing protein partner.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human MCC complex\nexperimental_model: Purified recombinant human MCC produced by baculovirus expression\nlimitations: Recombinant-enzyme kinetics do not determine human dietary requirements.\nexposure: ATP and methylcrotonyl-CoA kinetics\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/17360195.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3ae046490d9657d005d5c39efb1f7439bc015d0cb2190834e3e868de70eaf985\", \"start_char\": 0, \"end_char\": 1067, \"text_sha256\": \"3ae046490d9657d005d5c39efb1f7439bc015d0cb2190834e3e868de70eaf985\"}\n[b7-p17360195] Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). (2007). https://pubmed.ncbi.nlm.nih.gov/17360195/ DOI: 10.1016/j.pep.2007.01.012","model_system":"Purified recombinant human MCC produced by baculovirus expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p17360195] Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). (2007). https://pubmed.ncbi.nlm.nih.gov/17360195/ DOI: 10.1016/j.pep.2007.01.012","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9608806b-adb6-5a35-b042-057147135642","stable_key":"import-08ce9896-9d1c-5bbf-b705-5bfe771091d5","title":"Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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