{"id":"5049b3a4-ceff-5b5b-90da-ba6e5d65f8d9","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-mouse","predicate":"reduces_tested","statement":"Glycine at 5 mM reduced LDH release in mouse macrophages undergoing tested pyroptotic, necrotic and post-apoptotic lysis, with no additional protection in Ninj1-knockout cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e6bbcd91-c6e5-5d5c-9831-8f585dd07597","mechanism_event_label":"A late membrane-rupture step was inhibited across several injury models.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"f393510f-d561-5adc-be80-812cc035269f","slug":"mouse-macrophage-plasma-membrane-rupture","display_name":"Plasma membrane rupture in mouse macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e6bbcd91-c6e5-5d5c-9831-8f585dd07597","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-mouse-event","event_type":"observed_relationship","label":"A late membrane-rupture step was inhibited across several injury models.","description":"Glycine at 5 mM reduced LDH release in mouse macrophages undergoing tested pyroptotic, necrotic and post-apoptotic lysis, with no additional protection in Ninj1-knockout cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f393510f-d561-5adc-be80-812cc035269f","slug":"mouse-macrophage-plasma-membrane-rupture","display_name":"Plasma membrane rupture in mouse macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"984b9748-cec2-5eae-a9f6-d371c87042a4","slug":"mouse-ninj1","display_name":"Mouse ninjurin-1 / Ninj1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse iBMDM knockout and glycine comparisons; stimulus-specific exposure durations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No-additivity supports a shared process, not proof of direct glycine binding to NINJ1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A late membrane-rupture step was inhibited across several injury models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"02486f9c-64aa-50aa-884c-8e48f73100f5","evidence_kind":"source_excerpt","locator":"Lines 354-360","start_line":354,"end_line":360,"excerpt":"## glycine-ninj1-mouse\nA late membrane-rupture step was inhibited across several injury models.\nGlycine at 5 mM reduced LDH release in mouse macrophages undergoing tested pyroptotic, necrotic and post-apoptotic lysis, with no additional protection in Ninj1-knockout cells.\nModel: Mouse iBMDM knockout and glycine comparisons; stimulus-specific exposure durations.\nLimitations: No-additivity supports a shared process, not proof of direct glycine binding to NINJ1.\nEvidence access: Primary full text\nGlycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","model_system":"Mouse iBMDM knockout and glycine comparisons; stimulus-specific exposure durations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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