{"id":"50237668-ced6-58c4-8dbb-6565e7cd13b4","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-mouse-vlcad-rescue-limit","predicate":"partially-restores-enzyme-activity","statement":"FAD added to muscle assays only mildly improved depressed VLCAD activity in Slc25a32 mutant mice; VLCAD abundance was lower in K235R homozygotes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bfa792eb-769e-5fe2-b63d-7a852117194c","mechanism_event_label":"The long-chain oxidation enzyme also had limitations beyond immediately available free cofactor.","subject":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"object":{"id":"8760f61f-27a4-532b-bccc-b4ec5df85cb3","slug":"mouse-acadvl","display_name":"Mouse very-long-chain acyl-CoA dehydrogenase / Acadvl","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bfa792eb-769e-5fe2-b63d-7a852117194c","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-mouse-vlcad-rescue-limit-event","event_type":"biochemical_relationship","label":"The long-chain oxidation enzyme also had limitations beyond immediately available free cofactor.","description":"FAD added to muscle assays only mildly improved depressed VLCAD activity in Slc25a32 mutant mice; 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unverified.","entity":null}],"evidence":[{"id":"3ac6650e-2b9b-5324-94db-395d2cb6d749","evidence_kind":"source_excerpt","locator":"Lines 957-968","start_line":957,"end_line":968,"excerpt":"### b2-met-mouse-vlcad-rescue-limit\nFAD added to muscle assays only mildly improved depressed VLCAD activity in Slc25a32 mutant mice; VLCAD abundance was lower in K235R homozygotes.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The long-chain oxidation enzyme also had limitations beyond immediately available free cofactor.\norganism: Mus musculus\ntissue_or_cell_type: Skeletal-muscle homogenates\nexperimental_model: Slc25a32-null embryos and missense knock-in mice, isolated mitochondria and skeletal-muscle enzyme assays.\nlimitations: This does not establish the response of genetic ACADVL disease to riboflavin.\nexposure: Direct FAD addition; VLCAD measured separately from MCAD.\nevidence_spans: [{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC11072207\", \"locator\": \"HTML article p\", \"paragraph_index\": 50, \"char_start\": 0, \"char_end\": 1147, \"evidence_access\": \"full-text\"}]\n[peng-2022-slc25a32] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism (2022). https://pubmed.ncbi.nlm.nih.gov/35727412/ DOI: 10.1007/s00018-022-04404-0","model_system":"Slc25a32-null embryos and missense knock-in mice, isolated mitochondria and skeletal-muscle enzyme assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [peng-2022-slc25a32] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism (2022). https://pubmed.ncbi.nlm.nih.gov/35727412/ DOI: 10.1007/s00018-022-04404-0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}