{"id":"501989dd-98e5-5781-9541-682e0aed08d8","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-scavenging-peroxynitrite","predicate":"scavenges_in_assay","statement":"SAC scavenged peroxynitrite anion in the tested chemical system; IC50 was 0.80 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fdddc1b9-014f-58f3-8b0e-0fdfa8c1039b","mechanism_event_label":"Direct chemistry has a measured concentration requirement.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"4e699868-7078-52af-88ce-b9c0e295f675","slug":"peroxynitrite-anion","display_name":"Peroxynitrite anion","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fdddc1b9-014f-58f3-8b0e-0fdfa8c1039b","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-scavenging-peroxynitrite-event","event_type":"observed_relationship","label":"Direct chemistry has a measured concentration requirement.","description":"SAC scavenged peroxynitrite anion in the tested chemical system; IC50 was 0.80 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4e699868-7078-52af-88ce-b9c0e295f675","slug":"peroxynitrite-anion","display_name":"Peroxynitrite anion","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Assay-specific millimolar IC50 values","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC concentration series versus corresponding reference scavengers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reactive-species scavenging systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay potency is not an achieved human tissue concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Cell-free chemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Direct chemistry has a measured concentration requirement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[17576034] S-allylcysteine scavenges singlet oxygen and hypochlorous acid and protects LLC-PK(1) cells of potassium dichromate-induced toxicity. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17576034/ · DOI 10.1016/j.fct.2007.05.002","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Reactive-species scavenging systems","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e46c578-425a-56f0-986d-13f698540615","evidence_kind":"source_excerpt","locator":"Lines 104-111","start_line":104,"end_line":111,"excerpt":"## s-allylcysteine-scavenging-peroxynitrite\nDirect chemistry has a measured concentration requirement.\nSAC scavenged peroxynitrite anion in the tested chemical system; IC50 was 0.80 mM.\nModel: Reactive-species scavenging systems\nLimitations: Assay potency is not an achieved human tissue concentration.\nEvidence access: Primary abstract\n[17576034] S-allylcysteine scavenges singlet oxygen and hypochlorous acid and protects LLC-PK(1) cells of potassium dichromate-induced toxicity. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17576034/ · DOI 10.1016/j.fct.2007.05.002\nStructured context: {\"organism\": \"Cell-free chemical assays\", \"tissue_or_cell_type\": \"Reactive-species scavenging systems\", \"dose\": \"Assay-specific millimolar IC50 values\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"In vitro\", \"experimental_comparison\": \"SAC concentration series versus corresponding reference scavengers\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Reactive-species scavenging systems","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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