{"id":"50022c96-9998-5ec0-817d-65782f451471","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-rat-pituitary-lh","predicate":"increases_tested","statement":"Rat pituitary incubation with 0.1 or 1 mM D-aspartate increased measured LH and cGMP over 60 minutes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2ed8ec6c-0fd7-57da-bd68-8ae4d10e5917","mechanism_event_label":"A rat tissue experiment links the response to a second-messenger change.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"d05817e5-cc5d-55a3-8123-43e794e1aad5","slug":"rat-pituitary-lh-output","display_name":"LH content/output in rat pituitary incubation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2ed8ec6c-0fd7-57da-bd68-8ae4d10e5917","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-rat-pituitary-lh-event","event_type":"observed_relationship","label":"A rat tissue experiment links the response to a second-messenger change.","description":"Rat pituitary incubation with 0.1 or 1 mM D-aspartate increased measured LH and cGMP over 60 minutes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d05817e5-cc5d-55a3-8123-43e794e1aad5","slug":"rat-pituitary-lh-output","display_name":"LH content/output in rat pituitary incubation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6d2f509b-3a5b-5a38-b3e7-b148581edec9","slug":"cgmp","display_name":"Cyclic guanosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat pituitary; tissue plus medium assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The association does not alone prove cGMP is the exclusive causal mediator; not direct human pituitary evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A rat tissue experiment links the response to a second-messenger change.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19860889/ · DOI 10.1186/1477-7827-7-120","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e9d24b9-8c46-5bb7-8d59-e977ce2b0ff6","evidence_kind":"source_excerpt","locator":"Lines 368-374","start_line":368,"end_line":374,"excerpt":"## d-aspartate-rat-pituitary-lh\nA rat tissue experiment links the response to a second-messenger change.\nRat pituitary incubation with 0.1 or 1 mM D-aspartate increased measured LH and cGMP over 60 minutes.\nModel: Isolated rat pituitary; tissue plus medium assays.\nLimitations: The association does not alone prove cGMP is the exclusive causal mediator; not direct human pituitary evidence.\nEvidence access: Primary full text\nThe role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19860889/ · DOI 10.1186/1477-7827-7-120","model_system":"Isolated rat pituitary; tissue plus medium assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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