{"id":"4fe942e9-a76d-543f-89d6-f700bcc61f5d","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-creatine-rat-homocysteine","predicate":"reduced","statement":"Creatine feeding lowered plasma homocysteine by approximately 25% in the rat experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"befc07bf-6706-5a20-9d06-848da3bbda18","mechanism_event_label":"Supplying the finished product reduced a methylation-related blood marker in this animal setting.","subject":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"object":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"befc07bf-6706-5a20-9d06-848da3bbda18","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-creatine-rat-homocysteine-event","event_type":"biochemical_relationship","label":"Supplying the finished product reduced a methylation-related blood marker in this animal setting.","description":"Creatine feeding lowered plasma homocysteine by approximately 25% in the rat experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/11595668.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b195a8b17815bf7d9223831374e2f85ae2f90d8d74117ad7aa8d94af7a9e94b0\", \"start_char\": 0, \"end_char\": 1687, \"text_sha256\": \"b195a8b17815bf7d9223831374e2f85ae2f90d8d74117ad7aa8d94af7a9e94b0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Two-week feeding and isolated hepatocyte experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Guanidinoacetate or creatine feeding; guanidinoacetate plus methionine in hepatocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Animal methyl-demand experiment; effect sizes do not predict human homocysteine responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Supplying the finished product reduced a methylation-related blood marker in this animal setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p11595668] Methylation demand and homocysteine metabolism: effects of dietary provision of creatine and guanidinoacetate. (2001). https://pubmed.ncbi.nlm.nih.gov/11595668/ DOI: 10.1152/ajpendo.2001.281.5.e1095","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma and liver cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"18e38a02-a434-5444-b0dc-fb9f45f0df6c","evidence_kind":"source_excerpt","locator":"Lines 698-709","start_line":698,"end_line":709,"excerpt":"### creatine-creatine-rat-homocysteine\nCreatine feeding lowered plasma homocysteine by approximately 25% in the rat experiment.\nCondition category: normal\nnutrient_topic: Creatine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Supplying the finished product reduced a methylation-related blood marker in this animal setting.\norganism: Rats\ntissue_or_cell_type: Plasma and liver cells\nexperimental_model: Two-week feeding and isolated hepatocyte experiments\nlimitations: Animal methyl-demand experiment; effect sizes do not predict human homocysteine responses.\nexposure: Guanidinoacetate or creatine feeding; guanidinoacetate plus methionine in hepatocytes\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/11595668.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b195a8b17815bf7d9223831374e2f85ae2f90d8d74117ad7aa8d94af7a9e94b0\", \"start_char\": 0, \"end_char\": 1687, \"text_sha256\": \"b195a8b17815bf7d9223831374e2f85ae2f90d8d74117ad7aa8d94af7a9e94b0\"}\n[creatine-p11595668] Methylation demand and homocysteine metabolism: effects of dietary provision of creatine and guanidinoacetate. (2001). https://pubmed.ncbi.nlm.nih.gov/11595668/ DOI: 10.1152/ajpendo.2001.281.5.e1095","model_system":"Two-week feeding and isolated hepatocyte experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [creatine-p11595668] Methylation demand and homocysteine metabolism: effects of dietary provision of creatine and guanidinoacetate. (2001). https://pubmed.ncbi.nlm.nih.gov/11595668/ DOI: 10.1152/ajpendo.2001.281.5.e1095","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70358046-c5c3-5e9a-9fc9-eb01c3fff88b","stable_key":"import-44737fa3-b335-53f4-a644-b9878d4416ac","title":"Creatine: synthesis, transport, phosphocreatine energetics and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"548237d3e98a73a7dc02181807921fdd8b902adcee46248792258a761593eda9","revision_id":"88738255-8028-52fb-9c12-0fe92d8ce367","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}