{"id":"4fc528aa-e17a-534c-8f53-b62cda00b757","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tff3","predicate":"induces","statement":"CLA increased TFF3 in human SW480 cells; PPAR-gamma antagonist GW9662 prevented this induction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c400e609-af1f-503c-bfec-b3649806a3bd","mechanism_event_label":"A mucosal-repair protein responded through a receptor-sensitive pathway.","subject":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"object":{"id":"19e136bc-ca95-56fc-8009-d8f0f748d1c5","slug":"tff3","display_name":"Human trefoil factor 3 / TFF3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c400e609-af1f-503c-bfec-b3649806a3bd","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tff3-event","event_type":"observed_relationship","label":"A mucosal-repair protein responded through a receptor-sensitive pathway.","description":"CLA increased TFF3 in human SW480 cells; PPAR-gamma antagonist GW9662 prevented this induction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19e136bc-ca95-56fc-8009-d8f0f748d1c5","slug":"tff3","display_name":"Human trefoil factor 3 / TFF3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a8efc31d-682c-5f0b-b02c-acbdf7560139","slug":"gw9662","display_name":"GW9662","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colorectal cell line, 0–2.5 micromolar CLA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological support does not identify the active isomer or prove human mucosal healing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A mucosal-repair protein responded through a receptor-sensitive pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Dietary conjugated linoleic acid activates PPARγ and the intestinal trefoil factor in SW480 cells and mice with dextran sulfate sodium-induced colitis. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23077197/ · DOI 10.3945/jn.112.163931","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dfe278d3-02d2-5add-b263-2eeb163cc0ed","evidence_kind":"source_excerpt","locator":"Lines 374-380","start_line":374,"end_line":380,"excerpt":"## cla-tff3\nA mucosal-repair protein responded through a receptor-sensitive pathway.\nCLA increased TFF3 in human SW480 cells; PPAR-gamma antagonist GW9662 prevented this induction.\nModel: Human colorectal cell line, 0–2.5 micromolar CLA.\nLimitations: Pharmacological support does not identify the active isomer or prove human mucosal healing.\nEvidence access: Primary abstract\nDietary conjugated linoleic acid activates PPARγ and the intestinal trefoil factor in SW480 cells and mice with dextran sulfate sodium-induced colitis. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23077197/ · DOI 10.3945/jn.112.163931","model_system":"Human colorectal cell line, 0–2.5 micromolar CLA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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