{"id":"4fae1630-9815-5ce8-8b91-2316d9564fa9","stable_key":"research:txnrd3-sperm-thiol-control","predicate":"supports","statement":"Txnrd3 deletion in mice altered sperm protein thiol status, supporting a role for TXNRD3 in sperm redox remodeling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:supported_interpretation","direction":"positive","is_public":true,"mechanism_event_id":"51b1dc43-70d6-5367-9359-c819a1ceb871","mechanism_event_label":"TXNRD3 helps manage protein sulfur bonds as sperm mature.","subject":{"id":"bc046427-cd2b-50e4-a482-b37b08495514","slug":"txnrd3","display_name":"TXNRD3","entity_type_key":"protein"},"object":{"id":"3bb9271f-a26b-5685-9edf-47cb8ff087fd","slug":"sperm-thiol-homeostasis","display_name":"Sperm protein thiol homeostasis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"51b1dc43-70d6-5367-9359-c819a1ceb871","stable_key":"research:txnrd3-sperm-thiol-control","event_type":"experimentally_scoped_interaction","label":"TXNRD3 helps manage protein sulfur bonds as sperm mature.","description":"Txnrd3 deletion in mice altered sperm protein thiol status, supporting a role for TXNRD3 in sperm redox remodeling.","status":"active","compartment":null,"participants":[{"entity":{"id":"bc046427-cd2b-50e4-a482-b37b08495514","slug":"txnrd3","display_name":"TXNRD3","entity_type_key":"protein"},"role":"regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3bb9271f-a26b-5685-9edf-47cb8ff087fd","slug":"sperm-thiol-homeostasis","display_name":"Sperm protein thiol homeostasis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This experiment-specific relationship does not establish a human dietary-deficiency threshold or supplementation benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5a4d2910-db7b-5d63-9501-c34133ccd3b4","evidence_kind":"curated_literature_summary","locator":"lines 976-985","start_line":976,"end_line":985,"excerpt":"## txnrd3-sperm-thiol-control\n\nTXNRD3 helps manage protein sulfur bonds as sperm mature.\n\nTxnrd3 deletion in mice altered sperm protein thiol status, supporting a role for TXNRD3 in sperm redox remodeling.\n\nExperimental model: Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.\nOrganism: Mouse\nLimitations: This experiment-specific relationship does not establish a human dietary-deficiency threshold or supplementation benefit.\nPrimary reference: [Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis](https://pmc.ncbi.nlm.nih.gov/articles/PMC9352919/)","model_system":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"Reproduction","plain_language":"TXNRD3 helps manage protein sulfur bonds as sperm mature.","evidence_scope":"supported_interpretation","papers":[{"key":"catalog-txnrd3-2022","title":"Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9352919/","doi":null,"year":2022,"model":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","summary":"Txnrd3 deletion altered sperm thiol redox state and reproductive performance; the enzyme has thioredoxin/glutathione reductase activity in vitro."}]}}