{"id":"4f9b37f1-d1f5-5a3d-beaf-940483ea00c9","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:b3-redox-nadk2-knockdown-ros","predicate":"restrains","statement":"Three days after NADK2 knockdown, HEK293A intracellular ROS-probe signals increased with and without the tested menadione challenge.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5a98ca92-b9cd-5ad4-8430-1d651ba61ce7","mechanism_event_label":"Loss of the kinase increased a cellular oxidant readout in this experiment.","subject":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"object":{"id":"143e7c2e-4ee9-5dc0-9d61-afe3cc0c0a80","slug":"cellular-ros-assay-signal","display_name":"Cellular ROS assay signal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5a98ca92-b9cd-5ad4-8430-1d651ba61ce7","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:b3-redox-nadk2-knockdown-ros-event","event_type":"biochemical_relationship","label":"Loss of the kinase increased a cellular oxidant readout in this experiment.","description":"Three days after NADK2 knockdown, HEK293A intracellular ROS-probe signals increased with and without the tested menadione challenge.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"143e7c2e-4ee9-5dc0-9d61-afe3cc0c0a80","slug":"cellular-ros-assay-signal","display_name":"Cellular ROS assay signal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/niacin-redox-sources/nadk2-2012.fulltext.txt\", \"locator\": \"Results\", \"start_char\": 16830, \"end_char\": 18219, \"file_sha256\": \"65209c0b4ea7220bc95afdd182e40910f2445345172956e85552d5c6db649648\", \"text_sha256\": \"5de49f80d76c73b6a308096c07b330e8d3d53ee28c6a06f4b2a331e4798b45d7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"siRNA knockdown; CM-H2DCFDA readout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Three-day knockdown; vehicle or 100 μM menadione for 30 min in ROS assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CM-H2DCFDA is an intracellular probe; do not relabel this result as a direct measurement of mitochondrial ROS or assume dietary niacin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Niacin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"niacin","display_name":"Niacin (vitamin B3)","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of the kinase increased a cellular oxidant readout in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nadk2-2012] Identification and characterization of a human mitochondrial NAD kinase. (2012). https://pubmed.ncbi.nlm.nih.gov/23212377/ DOI: 10.1038/ncomms2262","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293A cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"cfbca7ac-3895-597e-bc09-01548bdf260a","evidence_kind":"source_excerpt","locator":"Lines 938-949","start_line":938,"end_line":949,"excerpt":"### b3-redox-nadk2-knockdown-ros\nThree days after NADK2 knockdown, HEK293A intracellular ROS-probe signals increased with and without the tested menadione challenge.\nCondition category: machinery_impairment\nnutrient_topic: Niacin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of the kinase increased a cellular oxidant readout in this experiment.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293A cells\nexperimental_model: siRNA knockdown; CM-H2DCFDA readout\nlimitations: CM-H2DCFDA is an intracellular probe; do not relabel this result as a direct measurement of mitochondrial ROS or assume dietary niacin deficiency.\nexposure: Three-day knockdown; vehicle or 100 μM menadione for 30 min in ROS assay\nevidence_span: {\"source_cache\": \"artifacts/niacin-redox-sources/nadk2-2012.fulltext.txt\", \"locator\": \"Results\", \"start_char\": 16830, \"end_char\": 18219, \"file_sha256\": \"65209c0b4ea7220bc95afdd182e40910f2445345172956e85552d5c6db649648\", \"text_sha256\": \"5de49f80d76c73b6a308096c07b330e8d3d53ee28c6a06f4b2a331e4798b45d7\"}\n[nadk2-2012] Identification and characterization of a human mitochondrial NAD kinase. 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