{"id":"4f8bdf77-ac9e-5c95-8985-0c51aa5fbc13","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-sulfasalazine-conjugase-inhibition","predicate":"inhibits","statement":"Sulfasalazine competitively inhibited human jejunal brush-border folate hydrolase, with reported Ki 0.13 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5e219121-b9bb-5b99-a993-8cdc6ea4622e","mechanism_event_label":"This drug can inhibit the folate digestion enzyme.","subject":{"id":"a5eafc6b-a85d-555d-9a24-13748e96218f","slug":"sulfasalazine","display_name":"Sulfasalazine","entity_type_key":"small_molecule"},"object":{"id":"f75b6059-4033-5997-8b92-acc37f81289d","slug":"folh1","display_name":"Human glutamate carboxypeptidase II / FOLH1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5e219121-b9bb-5b99-a993-8cdc6ea4622e","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-sulfasalazine-conjugase-inhibition-event","event_type":"biochemical_relationship","label":"This drug can inhibit the folate digestion enzyme.","description":"Sulfasalazine competitively inhibited human jejunal brush-border folate hydrolase, with reported Ki 0.13 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a5eafc6b-a85d-555d-9a24-13748e96218f","slug":"sulfasalazine","display_name":"Sulfasalazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f75b6059-4033-5997-8b92-acc37f81289d","slug":"folh1","display_name":"Human glutamate carboxypeptidase II / FOLH1","entity_type_key":"protein"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Partially purified human jejunal brush-border enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In-vitro inhibitor concentration series","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not quantify clinical malabsorption or establish every drug mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This drug can inhibit the folate digestion enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[reisenauer1981] Human jejunal brush border folate conjugase. Characteristics and inhibition by salicylazosulfapyridine (1981). https://pubmed.ncbi.nlm.nih.gov/6113848/ DOI: 10.1016/0005-2744(81)90271-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Jejunal brush border","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1bc6fcf5-bb06-5b63-b7c6-79664502057f","evidence_kind":"source_excerpt","locator":"Lines 94-104","start_line":94,"end_line":104,"excerpt":"### folate-sulfasalazine-conjugase-inhibition\nSulfasalazine competitively inhibited human jejunal brush-border folate hydrolase, with reported Ki 0.13 mM.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This drug can inhibit the folate digestion enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Jejunal brush border\nexperimental_model: Partially purified human jejunal brush-border enzyme\nlimitations: Does not quantify clinical malabsorption or establish every drug mechanism.\nexposure: In-vitro inhibitor concentration series\n[reisenauer1981] Human jejunal brush border folate conjugase. Characteristics and inhibition by salicylazosulfapyridine (1981). https://pubmed.ncbi.nlm.nih.gov/6113848/ DOI: 10.1016/0005-2744(81)90271-0","model_system":"Partially purified human jejunal brush-border enzyme","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [reisenauer1981] Human jejunal brush border folate conjugase. 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