{"id":"4f88bbbe-eeb6-5432-b64c-5eed00106916","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-gcpii-zinc-catalytic-center","predicate":"coordinates-catalytic-site-of","statement":"Human GCPII crystal structures resolved two zinc ions at the catalytic center, bridged by water or hydroxide and Asp387.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"04c3267c-fb8f-5c46-b6d0-ed348d40ad26","mechanism_event_label":"The folate-processing enzyme contains a two-zinc catalytic site.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"f75b6059-4033-5997-8b92-acc37f81289d","slug":"folh1","display_name":"Human glutamate carboxypeptidase II / FOLH1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"04c3267c-fb8f-5c46-b6d0-ed348d40ad26","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-gcpii-zinc-catalytic-center-event","event_type":"biochemical_relationship","label":"The folate-processing enzyme contains a two-zinc catalytic site.","description":"Human GCPII crystal structures resolved two zinc ions at the catalytic center, bridged by water or hydroxide and Asp387.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f75b6059-4033-5997-8b92-acc37f81289d","slug":"folh1","display_name":"Human glutamate carboxypeptidase II / FOLH1","entity_type_key":"protein"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"nutrient source","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"role":"physiological substrate class","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Zinc-folate: molecular catalytic-site dependency; dietary zinc deficiency was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human GCPII crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Glutamate, phosphate and inhibitor-bound structures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structural cofactor evidence does not establish zinc intake thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The folate-processing enzyme contains a two-zinc catalytic site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mesters2006] Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer (2006). https://pubmed.ncbi.nlm.nih.gov/16467855/ DOI: 10.1038/sj.emboj.7600969","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6b0b7b38-d583-5577-9d03-20e4adcc96a0","evidence_kind":"source_excerpt","locator":"Lines 118-129","start_line":118,"end_line":129,"excerpt":"### folate-gcpii-zinc-catalytic-center\nHuman GCPII crystal structures resolved two zinc ions at the catalytic center, bridged by water or hydroxide and Asp387.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The folate-processing enzyme contains a two-zinc catalytic site.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein\nexperimental_model: Recombinant human GCPII crystallography\nlimitations: Structural cofactor evidence does not establish zinc intake thresholds.\nexposure: Glutamate, phosphate and inhibitor-bound structures\ncross_nutrient: Zinc-folate: molecular catalytic-site dependency; dietary zinc deficiency was not tested.\n[mesters2006] Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer (2006). https://pubmed.ncbi.nlm.nih.gov/16467855/ DOI: 10.1038/sj.emboj.7600969","model_system":"Recombinant human GCPII crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mesters2006] Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer (2006). https://pubmed.ncbi.nlm.nih.gov/16467855/ DOI: 10.1038/sj.emboj.7600969","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}