{"id":"4f6955db-bb17-54ed-b74b-7a51b970a819","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ep300","predicate":"inhibits","statement":"Spermidine inhibited acetyltransferase activity of recombinant EP300 in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"110b2044-6c64-5ddb-9501-3d3e2332aa71","mechanism_event_label":"One route changes protein acetylation.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"9052514f-cb56-57bc-8df8-62b26b2ce3a2","slug":"ep300","display_name":"EP300","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"110b2044-6c64-5ddb-9501-3d3e2332aa71","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ep300-event","event_type":"observed_relationship","label":"One route changes protein acetylation.","description":"Spermidine inhibited acetyltransferase activity of recombinant EP300 in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9052514f-cb56-57bc-8df8-62b26b2ce3a2","slug":"ep300","display_name":"EP300","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human EP300 assay with companion human-cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical inhibition is not proof of selective target engagement after dietary exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One route changes protein acetylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine induces autophagy by inhibiting the acetyltransferase EP300. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25526088/ · DOI 10.1038/cdd.2014.215","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bbdde2a1-d822-5669-9303-8f52d5c8ebb5","evidence_kind":"source_excerpt","locator":"Lines 150-156","start_line":150,"end_line":156,"excerpt":"## spermidine-ep300\nOne route changes protein acetylation.\nSpermidine inhibited acetyltransferase activity of recombinant EP300 in vitro.\nModel: Recombinant human EP300 assay with companion human-cell experiments.\nLimitations: Biochemical inhibition is not proof of selective target engagement after dietary exposure.\nEvidence access: Primary abstract\nSpermidine induces autophagy by inhibiting the acetyltransferase EP300. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25526088/ · DOI 10.1038/cdd.2014.215","model_system":"Recombinant human EP300 assay with companion human-cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}