{"id":"4f2b3e52-6355-5bc8-94c2-d9f44c20e3cd","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-bcell-human","predicate":"improves","statement":"Spermidine supplementation restored the hypusination–TFEB–autophagy pathway and improved old-donor B-cell responses ex vivo.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc9ceb76-4c39-5c2c-a1b7-112565eeaa13","mechanism_event_label":"Older immune cells responded in culture.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"f510a828-6ff6-5ac7-8604-ec3788e21414","slug":"human-aged-b-cell-response","display_name":"Human older-donor B-cell response ex vivo","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fc9ceb76-4c39-5c2c-a1b7-112565eeaa13","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-bcell-human-event","event_type":"observed_relationship","label":"Older immune cells responded in culture.","description":"Spermidine supplementation restored the hypusination–TFEB–autophagy pathway and improved old-donor B-cell responses ex vivo.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f510a828-6ff6-5ac7-8604-ec3788e21414","slug":"human-aged-b-cell-response","display_name":"Human older-donor B-cell response ex vivo","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human B cells from older donors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not an oral vaccine-efficacy trial or proof of whole-body immune rejuvenation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Older immune cells responded in culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2c7aaad8-a495-5f0d-8f19-2ee230e69d8a","evidence_kind":"source_excerpt","locator":"Lines 230-236","start_line":230,"end_line":236,"excerpt":"## spermidine-bcell-human\nOlder immune cells responded in culture.\nSpermidine supplementation restored the hypusination–TFEB–autophagy pathway and improved old-donor B-cell responses ex vivo.\nModel: Primary human B cells from older donors.\nLimitations: Not an oral vaccine-efficacy trial or proof of whole-body immune rejuvenation.\nEvidence access: Primary full text\nPolyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","model_system":"Primary human B cells from older donors.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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