{"id":"4f03cdc5-87bd-5bea-9df8-a602e67aad80","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-tumor-transporter","predicate":"depletes_local_availability_for","statement":"Slc43a2 knockdown in B16F10/ID8 mouse tumor cells reduced methionine consumption and improved T-cell supply/function in coculture experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1993289d-7acc-5acd-9133-b407b27ae4c0","mechanism_event_label":"A neighboring cell can change supply without a dietary deficiency.","subject":{"id":"82eb202c-75eb-5682-be42-d7c94e5f00a3","slug":"mouse-slc43a2","display_name":"Mouse methionine transporter LAT4 / Slc43a2","entity_type_key":"protein"},"object":{"id":"a915b501-37d4-56d0-8ac6-a03792b3c969","slug":"mouse-tumor-tcell-methionine-competition","display_name":"Methionine competition between mouse tumor and T cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1993289d-7acc-5acd-9133-b407b27ae4c0","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-tumor-transporter-event","event_type":"observed_relationship","label":"A neighboring cell can change supply without a dietary deficiency.","description":"Slc43a2 knockdown in B16F10/ID8 mouse tumor cells reduced methionine consumption and improved T-cell supply/function in coculture experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"82eb202c-75eb-5682-be42-d7c94e5f00a3","slug":"mouse-slc43a2","display_name":"Mouse methionine transporter LAT4 / Slc43a2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a915b501-37d4-56d0-8ac6-a03792b3c969","slug":"mouse-tumor-tcell-methionine-competition","display_name":"Methionine competition between mouse tumor and T cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse B16F10/ID8 transporter-knockdown and T-cell coculture experiments; human expression correlations are distinct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Local competition cannot be inferred from plasma methionine alone. Correction record: Author correction published online 12 December 2025 (2026 issue): Extended Data Fig. 1j originally duplicated the A375-supernatant flow-cytometry plot in the Sup+Ser condition; publisher replaced the representative image. This is a figure correction, not an opposing scientific finding. https://www.nature.com/articles/s41586-025-09845-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A neighboring cell can change supply without a dietary deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cancer SLC43A2 alters T cell methionine metabolism and histone methylation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32879489/ · DOI 10.1038/s41586-020-2682-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"59ccbea4-f433-509d-a9be-63dc3d058535","evidence_kind":"source_excerpt","locator":"Lines 428-434","start_line":428,"end_line":434,"excerpt":"## methionine-tumor-transporter\nA neighboring cell can change supply without a dietary deficiency.\nSlc43a2 knockdown in B16F10/ID8 mouse tumor cells reduced methionine consumption and improved T-cell supply/function in coculture experiments.\nModel: Mouse B16F10/ID8 transporter-knockdown and T-cell coculture experiments; human expression correlations are distinct.\nLimitations: Local competition cannot be inferred from plasma methionine alone. Correction record: Author correction published online 12 December 2025 (2026 issue): Extended Data Fig. 1j originally duplicated the A375-supernatant flow-cytometry plot in the Sup+Ser condition; publisher replaced the representative image. This is a figure correction, not an opposing scientific finding. https://www.nature.com/articles/s41586-025-09845-8\nEvidence access: Primary abstract\nCancer SLC43A2 alters T cell methionine metabolism and histone methylation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32879489/ · DOI 10.1038/s41586-020-2682-1","model_system":"Mouse B16F10/ID8 transporter-knockdown and T-cell coculture experiments; human expression correlations are distinct.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f1c706ef72932eed85df234b6c3304158d503d626a63c69b02a453c162700d26","revision_id":"549035e0-3a72-550a-8a55-e8c5baa51b0d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}