{"id":"4efb09ac-f5d9-5fc6-ac89-2d82094f5d61","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-folate-no-organ-rescue","predicate":"does_not_change_in_trial","statement":"Randomized folic acid supplementation at 400 micrograms/day did not alter the response to the choline-depletion diet.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"def3de59-bc25-5f6f-9e77-efe80b7a88e6","mechanism_event_label":"Folic acid did not substitute for choline in preventing these liver or muscle endpoints.","subject":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"object":{"id":"57aaf728-3b0f-5dda-b2e4-69ddef823b6b","slug":"human-choline-depletion-organ-dysfunction","display_name":"Fatty liver or muscle damage during controlled human choline depletion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"def3de59-bc25-5f6f-9e77-efe80b7a88e6","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-folate-no-organ-rescue-event","event_type":"observed_intervention","label":"Folic acid did not substitute for choline in preventing these liver or muscle endpoints.","description":"Randomized folic acid supplementation at 400 micrograms/day did not alter the response to the choline-depletion diet.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"57aaf728-3b0f-5dda-b2e4-69ddef823b6b","slug":"human-choline-depletion-organ-dysfunction","display_name":"Fatty liver or muscle damage during controlled human choline depletion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/17490963.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28d18f06c2deb6f384ef2b5e84238b05deb076778619c21ee9682d49e34ae824\", \"start_char\": 0, \"end_char\": 1758, \"text_sha256\": \"28d18f06c2deb6f384ef2b5e84238b05deb076778619c21ee9682d49e34ae824\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Controlled depletion/repletion with randomized folic acid supplementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"550 mg choline/70 kg/day for 10 days, then less than 50 mg/70 kg/day up to 42 days; graded repletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supervised experimental depletion, not a recommended intake protocol. 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(2007). https://pubmed.ncbi.nlm.nih.gov/17490963/ DOI: 10.1093/ajcn/85.5.1275","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"57 adults: 26 men, 16 premenopausal and 15 postmenopausal women","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2b0257bf-e1cc-51fc-859e-fdb78b6047f1","evidence_kind":"source_excerpt","locator":"Lines 1023-1034","start_line":1023,"end_line":1034,"excerpt":"### choline-folate-no-organ-rescue\nRandomized folic acid supplementation at 400 micrograms/day did not alter the response to the choline-depletion diet.\nCondition category: nutrient_deficiency\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Folic acid did not substitute for choline in preventing these liver or muscle endpoints.\norganism: Human\ntissue_or_cell_type: 57 adults: 26 men, 16 premenopausal and 15 postmenopausal women\nexperimental_model: Controlled depletion/repletion with randomized folic acid supplementation\nlimitations: Supervised experimental depletion, not a recommended intake protocol. Liver/muscle endpoints and susceptibility differed; one cohort underlies related genetic publications.\nexposure: 550 mg choline/70 kg/day for 10 days, then less than 50 mg/70 kg/day up to 42 days; graded repletion\nevidence_span: {\"source_cache\": \"artifacts/choline-research/17490963.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28d18f06c2deb6f384ef2b5e84238b05deb076778619c21ee9682d49e34ae824\", \"start_char\": 0, \"end_char\": 1758, \"text_sha256\": \"28d18f06c2deb6f384ef2b5e84238b05deb076778619c21ee9682d49e34ae824\"}\n[choline-p17490963] Sex and menopausal status influence human dietary requirements for the nutrient choline. 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